RNA synthesis by nuclei and chromatin isolated from adult and senescent Wistar rat liver

G. H. Park, D. E. Buetow

Research output: Contribution to journalArticlepeer-review

Abstract

Nuclei isolated from senescent (22-26 months) female Wistar rat liver show a decreased RNA synthesis compared to nuclei from adult (12 months) liver. Kinetic analyses demonstrate a decreased rate (25-42%) and maximal incorporation (41-54%) of labeled uridine triphosphate into both rRNA and mRNA in senescent nuclei. Chromatin-bound RNA polymerase activity is decreased by 36%, whereas free RNA polymerase activity, i.e., not bound to chromatin, is increased by 41% in senescent nuclei, but the total bound plus free activity is the same in senescent and adult nuclei. Isolated senescent chromatin shows reduced transcriptional capacity and requires a higher temperature to initiate melting. A decreased ability of chromatin to bind RNA polymerases appears to underlie the observed decreased RNA synthesis in senescent liver nuclei.

Original languageEnglish (US)
Pages (from-to)61-75
Number of pages15
JournalGerontology
Volume36
Issue number2
DOIs
StatePublished - Jan 1 1990

Keywords

  • Chromatin
  • Liver
  • Nuclei
  • RNA synthesis

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Fingerprint Dive into the research topics of 'RNA synthesis by nuclei and chromatin isolated from adult and senescent Wistar rat liver'. Together they form a unique fingerprint.

Cite this