TY - JOUR
T1 - RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis
AU - Kaye, Emily G.
AU - Basavaraju, Kavyashree
AU - Nelson, Geoffrey M.
AU - Zomer, Helena D.
AU - Roy, Debarun
AU - Joseph, Irene Infancy
AU - Rajabi-Toustani, Reza
AU - Qiao, Huanyu
AU - Adelman, Karen
AU - Reddi, Prabhakara P.
N1 - The authors would like to thank the Nascent Transcriptomics Core at Harvard Medical School, Boston, MA for performing PRO-seq library construction. We also thank the HMS Biopolymers Facility, Bauer Core Facility at Harvard University and Novogene for sequencing. We are grateful to the DNA Services laboratory of the Roy J. Carver Biotechnology Center at the University of Illinois at Urbana-Champaign (UIUC) for scRNA-seq sample prep and sequencing. We acknowledge the services of the Histology Core of the College of Veterinary Medicine, UIUC. This work was supported by the National Institutes of Health (NIH R01HD36239 to P.P.R., R01GM135549 to H.Q., and NIH R01HD094546 to P.P.R. and K.A.)
The authors would like to thank the Nascent Transcriptomics Core at Harvard Medical School, Boston, MA for performing PRO-seq library construction. We also thank the HMS Biopolymers Facility, Bauer Core Facility at Harvard University and Novogene for sequencing. We are grateful to the DNA Services laboratory of the Roy J. Carver Biotechnology Center at the University of Illinois at Urbana-Champaign (UIUC) for scRNA-seq sample prep and sequencing. We acknowledge the services of the Histology Core of the College of Veterinary Medicine, UIUC. This work was supported by the National Institutes of Health (NIH R01HD36239 to P.P.R., R01GM135549 to H.Q., and NIH R01HD094546 to P.P.R. and K.A.)
PY - 2024/12
Y1 - 2024/12
N2 - Male germ cell development requires precise regulation of gene activity in a cell-type and stage-specific manner, with perturbations in gene expression during spermatogenesis associated with infertility. Here, we use steady-state, nascent and single-cell RNA sequencing strategies to comprehensively characterize gene expression across male germ cell populations, to dissect the mechanisms of gene control and provide new insights towards therapy. We discover a requirement for pausing of RNA Polymerase II (Pol II) at the earliest stages of sperm differentiation to establish the landscape of gene activity across development. Accordingly, genetic knockout of the Pol II pause-inducing factor NELF in immature germ cells blocks differentiation to spermatids. Further, we uncover unanticipated roles for Pol II pausing in the regulation of meiosis during spermatogenesis, with the presence of paused Pol II associated with double-strand break (DSB) formation, and disruption of meiotic gene expression and DSB repair in germ cells lacking NELF.
AB - Male germ cell development requires precise regulation of gene activity in a cell-type and stage-specific manner, with perturbations in gene expression during spermatogenesis associated with infertility. Here, we use steady-state, nascent and single-cell RNA sequencing strategies to comprehensively characterize gene expression across male germ cell populations, to dissect the mechanisms of gene control and provide new insights towards therapy. We discover a requirement for pausing of RNA Polymerase II (Pol II) at the earliest stages of sperm differentiation to establish the landscape of gene activity across development. Accordingly, genetic knockout of the Pol II pause-inducing factor NELF in immature germ cells blocks differentiation to spermatids. Further, we uncover unanticipated roles for Pol II pausing in the regulation of meiosis during spermatogenesis, with the presence of paused Pol II associated with double-strand break (DSB) formation, and disruption of meiotic gene expression and DSB repair in germ cells lacking NELF.
UR - http://www.scopus.com/inward/record.url?scp=85183397335&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85183397335&partnerID=8YFLogxK
U2 - 10.1038/s41467-024-45177-3
DO - 10.1038/s41467-024-45177-3
M3 - Article
C2 - 38287033
AN - SCOPUS:85183397335
SN - 2041-1723
VL - 15
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 848
ER -