RNA fluorescence in situ hybridization in cultured mammalian cells

Vidisha Tripathi; Jingyi Fei; Taekjip Ha; Kannanganattu V. Prasanth

doi:10.1007/978-1-4939-1369-5_11

RNA fluorescence in situ hybridization in cultured mammalian cells

Vidisha Tripathi
, Jingyi Fei
, Taekjip Ha
, Kannanganattu V. Prasanth

Research output: Contribution to journal › Article › peer-review

Abstract

It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

Original language	English (US)
Pages (from-to)	123-136
Number of pages	14
Journal	Methods in Molecular Biology
Volume	1206
DOIs	https://doi.org/10.1007/978-1-4939-1369-5_11
State	Published - 2015

Keywords

Fluorescence
In situ hybridization
Long noncoding RNA
RNA-FISH
lncRNA

ASJC Scopus subject areas

Molecular Biology
Genetics

Online availability

10.1007/978-1-4939-1369-5_11

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keywords = "Fluorescence, In situ hybridization, Long noncoding RNA, RNA-FISH, lncRNA",

author = "Vidisha Tripathi and Jingyi Fei and Taekjip Ha and Prasanth, \{Kannanganattu V.\}",

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AU - Tripathi, Vidisha

AU - Fei, Jingyi

AU - Ha, Taekjip

AU - Prasanth, Kannanganattu V.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

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Y1 - 2015

N2 - It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

AB - It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

KW - Fluorescence

KW - In situ hybridization

KW - Long noncoding RNA

KW - RNA-FISH

KW - lncRNA

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JF - Methods in Molecular Biology

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RNA fluorescence in situ hybridization in cultured mammalian cells

Abstract

Keywords

ASJC Scopus subject areas

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