RNA fluorescence in situ hybridization in cultured mammalian cells

Vidisha Tripathi; Jingyi Fei; Taekjip Ha; Kannanganattu V. Prasanth

doi:10.1007/978-1-4939-1369-5_11

RNA fluorescence in situ hybridization in cultured mammalian cells

Vidisha Tripathi, Jingyi Fei, Taekjip Ha, Kannanganattu V. Prasanth

Research output: Contribution to journal › Article › peer-review

Abstract

It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

Original language	English (US)
Pages (from-to)	123-136
Number of pages	14
Journal	Methods in Molecular Biology
Volume	1206
DOIs	https://doi.org/10.1007/978-1-4939-1369-5_11
State	Published - 2015

Keywords

Fluorescence
In situ hybridization
lncRNA
Long noncoding RNA
RNA-FISH

ASJC Scopus subject areas

Molecular Biology
Genetics

Online availability

10.1007/978-1-4939-1369-5_11

Library availability

Discover UIUC Full Text

Cite this

@article{4495cc8d91cf44559ccb6e345db367e8,

title = "RNA fluorescence in situ hybridization in cultured mammalian cells",

abstract = "It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.",

keywords = "Fluorescence, In situ hybridization, lncRNA, Long noncoding RNA, RNA-FISH",

author = "Vidisha Tripathi and Jingyi Fei and Taekjip Ha and Prasanth, {Kannanganattu V.}",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2015.",

year = "2015",

doi = "10.1007/978-1-4939-1369-5_11",

language = "English (US)",

volume = "1206",

pages = "123--136",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - RNA fluorescence in situ hybridization in cultured mammalian cells

AU - Tripathi, Vidisha

AU - Fei, Jingyi

AU - Ha, Taekjip

AU - Prasanth, Kannanganattu V.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

AB - It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

KW - Fluorescence

KW - In situ hybridization

KW - lncRNA

KW - Long noncoding RNA

KW - RNA-FISH

UR - http://www.scopus.com/inward/record.url?scp=84958663824&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84958663824&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-1369-5_11

DO - 10.1007/978-1-4939-1369-5_11

M3 - Article

C2 - 25240892

AN - SCOPUS:84958663824

SN - 1064-3745

VL - 1206

SP - 123

EP - 136

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

RNA fluorescence in situ hybridization in cultured mammalian cells

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this