RNA fluorescence in situ hybridization in cultured mammalian cells

Vidisha Tripathi, Jingyi Fei, Taekjip Ha, Prasanth Kumar Kannanganattu

Research output: Chapter in Book/Report/Conference proceedingChapter


It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

Original languageEnglish (US)
Title of host publicationRegulatory Non-Coding RNAs
Subtitle of host publicationMethods and Protocols
Number of pages14
ISBN (Electronic)9781493913695
ISBN (Print)9781493913688
StatePublished - 2015

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745


  • Fluorescence
  • In situ hybridization
  • Long noncoding RNA
  • lncRNA

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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