RNA extraction method from fruit tissue high in water and sugar

Angela R. Davis, Amnon Levi, Sungil Kim, Stephen R. King, Alvaro Hernandez

Research output: Contribution to journalArticlepeer-review

Abstract

RNA isolation from ripe fruit can be complicated by high concentrations of sugar and water. These sugars interfere with RNA extraction often resulting in low RNA quality and quantities, and high water concentrations dilute the RNA, making isolation difficult. We report a simple but novel method by which the majority of the excess sugar and water in mature fruit of tomato (Lycopersicon esculentum Mill.), watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai], and muskmelon (Cucumis melo L.) can be easily removed from tissue before RNA extraction. This method produced quality RNA in a shorter time than the currently accepted method for fruit tissue RNA isolation and does not require liquid nitrogen or a freeze dryer.

Original languageEnglish (US)
Pages (from-to)1292-1294
Number of pages3
JournalHortScience
Volume41
Issue number5
DOIs
StatePublished - Aug 2006

Keywords

  • Cucurbit
  • Liquid removal
  • RNA isolation
  • Rapid
  • Sugar removal
  • Tomato

ASJC Scopus subject areas

  • Horticulture

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