Rigid DNA chains near nanoparticles

Sangmin Jeon, Steve Granick

Research output: Contribution to journalArticle

Abstract

Two-photon excitation time-resolved fluorescence anisotropy and lifetime measurements were used to study the conformation of DNA near oppositely charged nanoparticles. Negatively-charged rhodamine-labeled DNA was allowed to adsorb onto positively-charged nanoparticles in deionized water and surface-induced fluorescence quenching was observed by measuring the fluorescence lifetime. Fluorescence quenching decreased with the addition of NaCl to the DNA-nanoparticle complex, which implies that the separation of DNA from the nanoparticles increased. However, comparison of fluorescence lifetime decays between free labeled-DNA and the DNA-nanoparticle complex shows that the adsorbed DNA remained less flexible than free labeled-DNA.

Original languageEnglish (US)
Pages (from-to)109-112
Number of pages4
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume238
Issue number1-3
DOIs
StatePublished - May 4 2004

Keywords

  • DNA
  • Fluorescence lifetime
  • Nanoparticle

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

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