RiboTag Analysis of Actively Translated mRNAs in Sertoli and Leydig Cells In Vivo

Elisenda Sanz, Ryan Evanoff, Albert Quintana, Elizabeth Evans, Jeremy A. Miller, Chemyong Ko, Paul S. Amieux, Michael D. Griswold, G. Stanley McKnight

Research output: Contribution to journalArticle

Abstract

Male spermatogenesis is a complex biological process that is regulated by hormonal signals from the hypothalamus (GnRH), the pituitary gonadotropins (LH and FSH) and the testis (androgens, inhibin). The two key somatic cell types of the testis, Leydig and Sertoli cells, respond to gonadotropins and androgens and regulate the development and maturation of fertilization competent spermatozoa. Although progress has been made in the identification of specific transcripts that are translated in Sertoli and Leydig cells and their response to hormones, efforts to expand these studies have been restricted by technical hurdles. In order to address this problem we have applied an in vivo ribosome tagging strategy (RiboTag) that allows a detailed and physiologically relevant characterization of the "translatome" (polysome-associated mRNAs) of Leydig or Sertoli cells in vivo. Our analysis identified all previously characterized Leydig and Sertoli cell-specific markers and identified in a comprehensive manner novel markers of Leydig and Sertoli cells; the translational response of these two cell types to gonadotropins or testosterone was also investigated. Modulation of a small subset of Sertoli cell genes occurred after FSH and testosterone stimulation. However, Leydig cells responded robustly to gonadotropin deprivation and LH restoration with acute changes in polysome-associated mRNAs. These studies identified the transcription factors that are induced by LH stimulation, uncovered novel potential regulators of LH signaling and steroidogenesis, and demonstrate the effects of LH on the translational machinery in vivo in the Leydig cell.

Original languageEnglish (US)
Article numbere66179
JournalPloS one
Volume8
Issue number6
DOIs
StatePublished - Jun 11 2013

Fingerprint

Leydig cells
Leydig Cells
Sertoli Cells
Sertoli cells
ribosomes
Gonadotropins
Ribosomes
Messenger RNA
Androgens
Testosterone
gonadotropins
Pituitary Gonadotropins
Inhibins
polyribosomes
Polyribosomes
Gonadotropin-Releasing Hormone
Restoration
Machinery
androgens
Transcription Factors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Sanz, E., Evanoff, R., Quintana, A., Evans, E., Miller, J. A., Ko, C., ... McKnight, G. S. (2013). RiboTag Analysis of Actively Translated mRNAs in Sertoli and Leydig Cells In Vivo. PloS one, 8(6), [e66179]. https://doi.org/10.1371/journal.pone.0066179

RiboTag Analysis of Actively Translated mRNAs in Sertoli and Leydig Cells In Vivo. / Sanz, Elisenda; Evanoff, Ryan; Quintana, Albert; Evans, Elizabeth; Miller, Jeremy A.; Ko, Chemyong; Amieux, Paul S.; Griswold, Michael D.; McKnight, G. Stanley.

In: PloS one, Vol. 8, No. 6, e66179, 11.06.2013.

Research output: Contribution to journalArticle

Sanz, E, Evanoff, R, Quintana, A, Evans, E, Miller, JA, Ko, C, Amieux, PS, Griswold, MD & McKnight, GS 2013, 'RiboTag Analysis of Actively Translated mRNAs in Sertoli and Leydig Cells In Vivo', PloS one, vol. 8, no. 6, e66179. https://doi.org/10.1371/journal.pone.0066179
Sanz, Elisenda ; Evanoff, Ryan ; Quintana, Albert ; Evans, Elizabeth ; Miller, Jeremy A. ; Ko, Chemyong ; Amieux, Paul S. ; Griswold, Michael D. ; McKnight, G. Stanley. / RiboTag Analysis of Actively Translated mRNAs in Sertoli and Leydig Cells In Vivo. In: PloS one. 2013 ; Vol. 8, No. 6.
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