Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

Joongoo Lee; Kevin J. Schwarz; Hao Yu; Antje Krüger; Eric V. Anslyn; Andrew D. Ellington; Jeffrey S. Moore; Michael C. Jewett

doi:10.1039/d0cc07740b

Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

Joongoo Lee
, Kevin J. Schwarz
, Hao Yu
, Antje Krüger
, Eric V. Anslyn
, Andrew D. Ellington
, Jeffrey S. Moore
, Michael C. Jewett

Research output: Contribution to journal › Article › peer-review

Abstract

We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.

Original language	English (US)
Pages (from-to)	2661-2664
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	21
Early online date	2021
DOIs	https://doi.org/10.1039/d0cc07740b
State	Published - Mar 14 2021

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d0cc07740b

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title = "Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro",

abstract = "We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.",

author = "Joongoo Lee and Schwarz, \{Kevin J.\} and Hao Yu and Antje Kr{\"u}ger and Anslyn, \{Eric V.\} and Ellington, \{Andrew D.\} and Moore, \{Jeffrey S.\} and Jewett, \{Michael C.\}",

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doi = "10.1039/d0cc07740b",

language = "English (US)",

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T1 - Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

AU - Lee, Joongoo

AU - Schwarz, Kevin J.

AU - Yu, Hao

AU - Krüger, Antje

AU - Anslyn, Eric V.

AU - Ellington, Andrew D.

AU - Moore, Jeffrey S.

AU - Jewett, Michael C.

N1 - JL, KJS, HY, AK, EVA, ADE, JSM, and MCJ conceived and designed the research. JL, KJS, HY, and AK performed experiments. All authors wrote and edited the manuscript. JS M and MCJ supervised this study. This work was supported by the Army Research Office Grant W911NF-16-1-0372. This work made use of IMSERC at Northwestern University.

PY - 2021/3/14

Y1 - 2021/3/14

N2 - We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.

AB - We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.

UR - https://www.scopus.com/pages/publications/85102351901

UR - https://www.scopus.com/pages/publications/85102351901#tab=citedBy

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DO - 10.1039/d0cc07740b

M3 - Article

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AN - SCOPUS:85102351901

SN - 1359-7345

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SP - 2661

EP - 2664

JO - Chemical Communications

JF - Chemical Communications

IS - 21

ER -

Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

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