Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

Joongoo Lee; Kevin J. Schwarz; Hao Yu; Antje Krüger; Eric V. Anslyn; Andrew D. Ellington; Jeffrey S. Moore; Michael C. Jewett

doi:10.1039/d0cc07740b

Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

Joongoo Lee, Kevin J. Schwarz, Hao Yu, Antje Krüger, Eric V. Anslyn, Andrew D. Ellington, Jeffrey S. Moore, Michael C. Jewett

Research output: Contribution to journal › Article › peer-review

Abstract

We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.

Original language	English (US)
Pages (from-to)	2661-2664
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	21
DOIs	https://doi.org/10.1039/d0cc07740b
State	Published - Mar 14 2021

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d0cc07740b

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AU - Lee, Joongoo

AU - Schwarz, Kevin J.

AU - Yu, Hao

AU - Krüger, Antje

AU - Anslyn, Eric V.

AU - Ellington, Andrew D.

AU - Moore, Jeffrey S.

AU - Jewett, Michael C.

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AB - We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.

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Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

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