Ribosome bypassing at serine codons as a test of the model of selective transfer RNA charging

Dale Lindsley; Paul Bonthuis; Jonathan Gallant; Teodora Tofoleanu; Johan Elf; Måns Ehrenberg

doi:10.1038/sj.embor.7400332

Ribosome bypassing at serine codons as a test of the model of selective transfer RNA charging

Dale Lindsley, Paul Bonthuis, Jonathan Gallant, Teodora Tofoleanu, Johan Elf, Måns Ehrenberg

Research output: Contribution to journal › Article › peer-review

Abstract

Recently, a model of the flux of amino acids through transfer RNAs (tRNAs) and into protein has been developed. The model predicts that the charging level of different isoacceptors carrying the same amino acid respond very differently to variation in supply of the amino acid or of the rate of charging. It has also been shown that ribosome bypassing is specifically stimulated at 'hungry' codons calling for an aminoacyl-tRNA in short supply. We have constructed two reporters of bypassing, which differ only in the identity of the serine codon subjected to starvation. The stimulation of bypassing as a function of starvation differed greatly between the two serine codons, in good agreement with the quantitative predictions of the model.

Original language	English (US)
Pages (from-to)	147-150
Number of pages	4
Journal	EMBO Reports
Volume	6
Issue number	2
DOIs	https://doi.org/10.1038/sj.embor.7400332
State	Published - Mar 15 2005
Externally published	Yes

Keywords

Amino acid
Bypassing
Charging
Protein synthesis
tRNA

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

Online availability

10.1038/sj.embor.7400332

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AU - Bonthuis, Paul

AU - Gallant, Jonathan

AU - Tofoleanu, Teodora

AU - Elf, Johan

AU - Ehrenberg, Måns

PY - 2005/3/15

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AB - Recently, a model of the flux of amino acids through transfer RNAs (tRNAs) and into protein has been developed. The model predicts that the charging level of different isoacceptors carrying the same amino acid respond very differently to variation in supply of the amino acid or of the rate of charging. It has also been shown that ribosome bypassing is specifically stimulated at 'hungry' codons calling for an aminoacyl-tRNA in short supply. We have constructed two reporters of bypassing, which differ only in the identity of the serine codon subjected to starvation. The stimulation of bypassing as a function of starvation differed greatly between the two serine codons, in good agreement with the quantitative predictions of the model.

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Ribosome bypassing at serine codons as a test of the model of selective transfer RNA charging

Abstract

Keywords

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