Review and Hypothesis. New insights into the reaction mechanism of transhydrogenase: Swivelling the dIII component may gate the proton channel

J. Baz Jackson, Josephine H. Leung, Charles D. Stout, Lici A. Schurig-Briccio, Robert B. Gennis

Research output: Contribution to journalReview article

Abstract

The membrane protein transhydrogenase in animal mitochondria and bacteria couples reduction of NADP + by NADH to proton translocation. Recent X-ray data on Thermus thermophilus transhydrogenase indicate a significant difference in the orientations of the two dIII components of the enzyme dimer (Leung et al., 2015). The character of the orientation change, and a review of information on the kinetics and thermodynamics of transhydrogenase, indicate that dIII swivelling might assist in the control of proton gating by the redox state of bound NADP + /NADPH during enzyme turnover.

Original languageEnglish (US)
Pages (from-to)2027-2033
Number of pages7
JournalFEBS Letters
Volume589
Issue number16
DOIs
StatePublished - Jul 20 2015

Fingerprint

NADP
Protons
Thermus thermophilus
Mitochondria
Enzymes
Thermodynamics
Dimers
NAD
Oxidation-Reduction
Bacteria
Membrane Proteins
Animals
X-Rays
X rays
Kinetics

Keywords

  • Membrane-protein structure
  • Nicotinamide nucleotide
  • Proton-gating
  • Proton-pump
  • Transhydrogenase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Review and Hypothesis. New insights into the reaction mechanism of transhydrogenase : Swivelling the dIII component may gate the proton channel. / Jackson, J. Baz; Leung, Josephine H.; Stout, Charles D.; Schurig-Briccio, Lici A.; Gennis, Robert B.

In: FEBS Letters, Vol. 589, No. 16, 20.07.2015, p. 2027-2033.

Research output: Contribution to journalReview article

Jackson, J. Baz ; Leung, Josephine H. ; Stout, Charles D. ; Schurig-Briccio, Lici A. ; Gennis, Robert B. / Review and Hypothesis. New insights into the reaction mechanism of transhydrogenase : Swivelling the dIII component may gate the proton channel. In: FEBS Letters. 2015 ; Vol. 589, No. 16. pp. 2027-2033.
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