Amplificación isotérmica mediada por bucle de transcripción inversa y protocolos alternativos para pruebas COVID-19 a gran escala y de bajo costo: lecciones de una economía emergente

Translated title of the contribution: Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy

Ismael Segura-Ulate, Alejandro Bolívar-González, Germán Madrigal-Redondo, Santiago Nuñez-Corrales, Andrés Gatica-Arias

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: Most successful cases of COVID-19 pandemic mitigation and handling have relied on extensive reverse-transcription quantitative polymerase chain reaction (RT-qPCR). However, many emerging economies have struggled with current molecular testing demands due to economic, technical and technological constraints. Objective: To define a potential diagnostic protocol to increase testing capacity in current and post-pandemic conditions. Methods: We reviewed the literature, patents and commercial applications, for alternatives. Results: We found a good potential in saliva samples, viral inactivation and quick RNA extraction by heating; the use of an isothermal technology such as loop mediated isothermal amplification (LAMP) and naked eye test-result visualization by in-tube colorimetry or turbidity. Conclusions: Saliva samples with quick RNA extraction by heating and colorimetric LAMP are promising options for countries with economic and infrastructure limitations.

Translated title of the contributionReverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy
Original languageSpanish
Pages (from-to)173-189
Number of pages17
JournalRevista de Biologia Tropical
Volume70
Issue number1
DOIs
StatePublished - Jan 1 2022

Keywords

  • emerging economies
  • loop mediated isothermal amplification
  • molecular testing
  • saliva
  • SARS-CoV-2

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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