Reverse genetics for porcine reproductive and respiratory syndrome virus

Mingyuan Han, Hanzhong Ke, Yijun Du, Qingzhan Zhang, Dongwan Yoo

Research output: Chapter in Book/Report/Conference proceedingChapter


Porcine reproductive and respiratory syndrome (PRRS) is a reemerging swine disease, and has become economically the most significant disease in pork production worldwide. The causative agent is PRRS virus (PRRSV), which is a member virus of the family Arteriviridae. The PRRSV genome is a single-stranded positive-sense RNA and is infectious. Two strategies in the PRRSV reverse genetics system have been employed for reconstitution of progeny virus: RNA transfection and DNA transfection. The PRRSV reverse genetics has broadly been used for studies including protein structure-function relationship, foreign gene expression, vaccine development, virulence determinants, and viral pathogenesis. Herein, we describe the modification of the pFL12 “RNA launch” reverse genetic system to the CMV promoter-driven pXJ41-FL13 “DNA launch” system. The generation of progeny PRRSV using pXJ41-FL13 is further elucidated.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages18
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Arterivirus
  • Infectious clones
  • Nidovirus
  • PRRS
  • Porcine reproductive and respiratory syndrome virus
  • Reverse genetics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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