Reversal of the β-oxidation cycle in saccharomyces cerevisiae for production of fuels and chemicals

Jiazhang Lian, Huimin Zhao

Research output: Contribution to journalArticle

Abstract

Functionally reversing the β-oxidation cycle represents an efficient and versatile strategy for synthesis of a wide variety of fuels and chemicals. However, due to the compartmentalization of cellular metabolisms, reversing the β-oxidation cycle in eukaryotic systems remains elusive. Here, we report the first successful reversal of the β-oxidation cycle in Saccharomyces cerevisiae, an important cell factory for large-scale production of fuels and chemicals. After extensive gene cloning and enzyme activity assays, a reversed β-oxidation pathway was functionally constructed in the yeast cytosol, which led to the synthesis of n-butanol, medium-chain fatty acids (MCFAs), and medium-chain fatty acid ethyl esters (MCFAEEs). The resultant recombinant strain provides a new broadly applicable platform for synthesis of fuels and chemicals in S. Cerevisiae.

Original languageEnglish (US)
Pages (from-to)332-341
Number of pages10
JournalACS synthetic biology
Volume4
Issue number3
DOIs
StatePublished - Mar 20 2015

Keywords

  • advanced biofuels
  • fatty acid
  • synthetic biology
  • yeast
  • β-oxidation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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