Restoration of growth phenotypes of Escherichia coli DH5α in minimal media through reversal of a point mutation in purB

Suk Chae Jung; Chris L. Smith; Ki Sung Lee; Min Eui Hong; Dae Hyuk Kweon; Gregory Stephanopoulos; Yong Su Jin

doi:10.1128/AEM.01210-10

Restoration of growth phenotypes of Escherichia coli DH5α in minimal media through reversal of a point mutation in purB

Suk Chae Jung, Chris L. Smith, Ki Sung Lee, Min Eui Hong, Dae Hyuk Kweon, Gregory Stephanopoulos, Yong Su Jin

Research output: Contribution to journal › Article › peer-review

Abstract

A point mutation (E115K) resulting in slower growth of Escherichia coli DH5α and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.

Original language	English (US)
Pages (from-to)	6307-6309
Number of pages	3
Journal	Applied and environmental microbiology
Volume	76
Issue number	18
DOIs	https://doi.org/10.1128/AEM.01210-10
State	Published - Sep 2010

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/AEM.01210-10

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title = "Restoration of growth phenotypes of Escherichia coli DH5α in minimal media through reversal of a point mutation in purB",

abstract = "A point mutation (E115K) resulting in slower growth of Escherichia coli DH5α and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.",

author = "Jung, {Suk Chae} and Smith, {Chris L.} and Lee, {Ki Sung} and Hong, {Min Eui} and Kweon, {Dae Hyuk} and Gregory Stephanopoulos and Jin, {Yong Su}",

year = "2010",

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doi = "10.1128/AEM.01210-10",

language = "English (US)",

volume = "76",

pages = "6307--6309",

journal = "Applied and environmental microbiology",

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publisher = "American Society for Microbiology",

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AU - Jung, Suk Chae

AU - Smith, Chris L.

AU - Lee, Ki Sung

AU - Hong, Min Eui

AU - Kweon, Dae Hyuk

AU - Stephanopoulos, Gregory

AU - Jin, Yong Su

PY - 2010/9

Y1 - 2010/9

N2 - A point mutation (E115K) resulting in slower growth of Escherichia coli DH5α and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.

AB - A point mutation (E115K) resulting in slower growth of Escherichia coli DH5α and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.

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JO - Applied and environmental microbiology

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IS - 18

ER -

Restoration of growth phenotypes of Escherichia coli DH5α in minimal media through reversal of a point mutation in purB

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