Response of enzymes and storage proteins of maize endosperm to nitrogen supply

George W. Singletary, Douglas C. Doehlert, Curtis M. Wilson, Michael J. Muhitch, Frederick E. Below

Research output: Contribution to journalArticle

Abstract

To examine the effects of N nutrition upon endosperm development, maize (Zea mays) kernels were grown in vitro with either 0, 3.6, 7.1, 14.3, or 35.7 millimolar N. Kernels were harvested at 20 days after pollination for determination of enzyme activities and again at maturity for quantification of storage products and electrophoretic separation of zeins. Endosperm dry weight, starch, zein-N, and nonzein-N all increased in mature kernels as N supply increased from zero to 14.3 millimolar. The activities of sucrose synthase, aldolase, phosphoglucomutase, glutamate-pyruvate transaminase, glutamate-oxaloacetate transaminase, and acetolactate synthase increased from 1- to 2.5-fold with increasing N supply. Adenosine diphosphate-glucose pyrophosphorylase and both ATP- and PPi-dependent phosphofructokinases increased to lesser extents, while no significant response was detected for hexose kinases and glutamine synthetase. Nitrogen-induced changes in enzyme activities were often highly correlated with changes in final starch and/or zein-N contents. Separation of zeins indicated that these peptides were proportionately enhanced by N supply, with the exception of C-zein, which was relatively insensitive to N. These data indicate that at least a portion of the yield increase in maize produced by N fertilization is induced by a modification of kernel metabolism in response to N supply.

Original languageEnglish (US)
Pages (from-to)858-864
Number of pages7
JournalPlant physiology
Volume94
Issue number3
DOIs
StatePublished - Jan 1 1990

Fingerprint

Zein
Endosperm
storage proteins
zein
endosperm
Zea mays
Nitrogen
corn
nitrogen
Enzymes
enzymes
seeds
Transaminases
Proteins
Starch
starch
enzyme activity
Glutamic Acid
phosphoglucomutase
Glucose-1-Phosphate Adenylyltransferase

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Response of enzymes and storage proteins of maize endosperm to nitrogen supply. / Singletary, George W.; Doehlert, Douglas C.; Wilson, Curtis M.; Muhitch, Michael J.; Below, Frederick E.

In: Plant physiology, Vol. 94, No. 3, 01.01.1990, p. 858-864.

Research output: Contribution to journalArticle

Singletary, GW, Doehlert, DC, Wilson, CM, Muhitch, MJ & Below, FE 1990, 'Response of enzymes and storage proteins of maize endosperm to nitrogen supply', Plant physiology, vol. 94, no. 3, pp. 858-864. https://doi.org/10.1104/pp.94.3.858
Singletary, George W. ; Doehlert, Douglas C. ; Wilson, Curtis M. ; Muhitch, Michael J. ; Below, Frederick E. / Response of enzymes and storage proteins of maize endosperm to nitrogen supply. In: Plant physiology. 1990 ; Vol. 94, No. 3. pp. 858-864.
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