Resolving postglacial phylogeography using high-throughput sequencing

Kevin J. Emerson, Clayton R. Merz, Julian M. Catchen, Paul A. Hohenlohe, William A. Cresko, William E. Bradshaw, Christina M. Holzapfel

Research output: Contribution to journalArticlepeer-review


The distinction between model and nonmodel organisms is becoming increasingly blurred. High-throughput, second-generation sequencing approaches are being applied to organisms based on their interesting ecological, physiological, developmental, or evolutionary properties and not on the depth of genetic information available for them. Here, we illustrate this point using a low-cost, efficient technique to determine the fine-scale phylogenetic relationships among recently diverged populations in a species. This application of restriction site-associated DNA tags (RAD tags) reveals previously unresolved genetic structure and direction of evolution in the pitcher plant mosquito, Wyeomyia smithii, from a southern Appalachian Mountain refugium following recession of the Laurentide Ice Sheet at 22,000-19,000 B.P. The RAD tag method can be used to identify detailed patterns of phylogeography in any organism regardless of existing genomic data, and, more broadly, to identify incipient speciation and genome-wide variationin natural populations in general.

Original languageEnglish (US)
Pages (from-to)16196-16200
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number37
StatePublished - Sep 14 2010
Externally publishedYes


  • Genomics
  • Restriction site-associated DNA tag
  • Second-generation sequencing
  • Wyeomyia smithii

ASJC Scopus subject areas

  • General


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