Resolution of two molecular forms of sucrose-phosphate synthase from maize, soybean and spinach leaves

Phillip S. Kerr, Willy Kalt-Torres, Steven C Huber

Research output: Contribution to journalArticle

Abstract

Two forms of sucrose-phosphate synthase (EC 2.4.1.14) were resolved from leaves of three species, maize (Zea mays L. cv. Pioneer 3184), soybean (Glycine max (L.) Merr., cv. Ransom) and spinach (Spinacia oleracea L. cv. Resistoflay) by hydroxyapatite Ultrogel chromatography, using a 75-mM (designated peak 1) and 250-mM (peak 2) K-phosphate discontinuous-gradient elution. Rechromatography of the two forms showed that they were not readily interconvertible. The distribution of activity between the two forms differed among species and changed during purification of the enzyme. Recovery of peak-1 activity was specifically lowered when maize leaf extracts were prepared in the absence of magnesium, indicating that the two forms may differ in stability. In addition, the forms of the enzyme from maize differed in the extent of glucose-6-phosphate activation. These results provide evidence for the existence of multiple forms of sucrose-phosphate synthase in leaves of different species and that the forms differ in regulatory properties.

Original languageEnglish (US)
Pages (from-to)515-519
Number of pages5
JournalPlanta
Volume170
Issue number4
DOIs
StatePublished - Apr 1 1987

Fingerprint

sucrose-phosphate synthase
Spinacia oleracea
Soybeans
spinach
Zea mays
soybeans
corn
leaves
hydroxyapatite
glucose 6-phosphate
enzymes
leaf extracts
Glucose-6-Phosphate
Glycine max
chromatography
magnesium
Enzymes
Durapatite
phosphates
Magnesium

Keywords

  • Glycine (sucrose-phosphate synthase)
  • Spinacia
  • Sucrose-phosphate synthase
  • Zea (sucrose-phosphate synthase)

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

Resolution of two molecular forms of sucrose-phosphate synthase from maize, soybean and spinach leaves. / Kerr, Phillip S.; Kalt-Torres, Willy; Huber, Steven C.

In: Planta, Vol. 170, No. 4, 01.04.1987, p. 515-519.

Research output: Contribution to journalArticle

Kerr, Phillip S. ; Kalt-Torres, Willy ; Huber, Steven C. / Resolution of two molecular forms of sucrose-phosphate synthase from maize, soybean and spinach leaves. In: Planta. 1987 ; Vol. 170, No. 4. pp. 515-519.
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