Resolution of spatial constraints during replication of peripheral chromatin

O. A. Zhironkina, S. Yu Kurchashova, A. L. Bratseva, V. D. Cherepanynets, O. S. Strelkova, A. S. Belmont, I. I. Kireev

Research output: Contribution to journalArticlepeer-review


Tight association of peripheral chromatin with nuclear lamina unavoidably creates topological constraints during replication. Additional complications are associated with high stability of lamina meshwork, which may hinder an access of replication factors to the sites of DNA synthesis in highly condensed template with limited mobility. In the current work we studied structural organization and dynamics of lamina as a function of replicative status of associated peripheral heterochromatin. The studies of molecular mobility of laminas at various stages of S-phase in vivo and using super-resolution microscopy showed no correlation between lamina dynamics and replicative status of attached heterochromatin. These data support the hypothesis that lamina-chromatin interactions during S-phase are regulated at the level of adapter proteins. Ultrastructural studies have demonstrated that temporal break of lamina-chromatin connections during replication does not cause noticeable spatial separation of replicating domains from nuclear periphery.

Original languageEnglish (US)
Pages (from-to)899-906
Number of pages8
Issue number12
StatePublished - 2014
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology


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