Resolution of allelic and non-allelic variants of histone H1 by cation-exchange-hydrophilic-interaction chromatography

Craig A. Mizzen; Andrew J. Alpert; Lyne Lévesque; Theo P.A. Kruck; Donald R. McLachlan

doi:10.1016/S0378-4347(00)00210-3

Resolution of allelic and non-allelic variants of histone H1 by cation-exchange-hydrophilic-interaction chromatography

Craig A. Mizzen, Andrew J. Alpert, Lyne Lévesque, Theo P.A. Kruck, Donald R. McLachlan

Research output: Contribution to journal › Article › peer-review

Abstract

A mixed-mode high-performance liquid chromatography (HPLC) method that resolves the six known non-allelic variants of chicken erythrocyte histone H1 is described. Common, but previously unknown, allelic variants of H1 that comigrate in polyacrylamide gel electrophoresis are also resolved. The resolution of H1 variants achieved by this method should be useful in determining the functional significance of H1 sequence heterogeneity and in analyses of post-translational modification of H1. Furthermore, the principles behind the separation should be applicable to analyses of polymorphism in other proteins. Copyright (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	33-46
Number of pages	14
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	744
Issue number	1
DOIs	https://doi.org/10.1016/S0378-4347(00)00210-3
State	Published - Jul 7 2000
Externally published	Yes

Keywords

Histone H1

ASJC Scopus subject areas

General Chemistry

Online availability

10.1016/S0378-4347(00)00210-3

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title = "Resolution of allelic and non-allelic variants of histone H1 by cation-exchange-hydrophilic-interaction chromatography",

abstract = "A mixed-mode high-performance liquid chromatography (HPLC) method that resolves the six known non-allelic variants of chicken erythrocyte histone H1 is described. Common, but previously unknown, allelic variants of H1 that comigrate in polyacrylamide gel electrophoresis are also resolved. The resolution of H1 variants achieved by this method should be useful in determining the functional significance of H1 sequence heterogeneity and in analyses of post-translational modification of H1. Furthermore, the principles behind the separation should be applicable to analyses of polymorphism in other proteins. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Histone H1",

author = "Mizzen, {Craig A.} and Alpert, {Andrew J.} and Lyne L{\'e}vesque and Kruck, {Theo P.A.} and McLachlan, {Donald R.}",

note = "Funding Information: We thank Dr. Paul Fraser for assistance with CD spectroscopy. This work was financed by grants from the Ontario Mental Health Foundation and the Hunter Foundation.",

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doi = "10.1016/S0378-4347(00)00210-3",

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T1 - Resolution of allelic and non-allelic variants of histone H1 by cation-exchange-hydrophilic-interaction chromatography

AU - Mizzen, Craig A.

AU - Alpert, Andrew J.

AU - Lévesque, Lyne

AU - Kruck, Theo P.A.

AU - McLachlan, Donald R.

N1 - Funding Information: We thank Dr. Paul Fraser for assistance with CD spectroscopy. This work was financed by grants from the Ontario Mental Health Foundation and the Hunter Foundation.

PY - 2000/7/7

Y1 - 2000/7/7

N2 - A mixed-mode high-performance liquid chromatography (HPLC) method that resolves the six known non-allelic variants of chicken erythrocyte histone H1 is described. Common, but previously unknown, allelic variants of H1 that comigrate in polyacrylamide gel electrophoresis are also resolved. The resolution of H1 variants achieved by this method should be useful in determining the functional significance of H1 sequence heterogeneity and in analyses of post-translational modification of H1. Furthermore, the principles behind the separation should be applicable to analyses of polymorphism in other proteins. Copyright (C) 2000 Elsevier Science B.V.

AB - A mixed-mode high-performance liquid chromatography (HPLC) method that resolves the six known non-allelic variants of chicken erythrocyte histone H1 is described. Common, but previously unknown, allelic variants of H1 that comigrate in polyacrylamide gel electrophoresis are also resolved. The resolution of H1 variants achieved by this method should be useful in determining the functional significance of H1 sequence heterogeneity and in analyses of post-translational modification of H1. Furthermore, the principles behind the separation should be applicable to analyses of polymorphism in other proteins. Copyright (C) 2000 Elsevier Science B.V.

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Resolution of allelic and non-allelic variants of histone H1 by cation-exchange-hydrophilic-interaction chromatography

Abstract

Keywords

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