Resolution enhancement in solid-state NMR of oriented membrane proteins by anisotropic differential linebroadening

Thomas Vosegaard, Kresten Bertelsen, Jan M. Pedersen, Lea Thøgersen, Birgit Schiøtt, Emad Tajkhorshid, Troels Skrydstrup, Niels Chr Nielsen

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate that a significant improvement in the spectral resolution may be achieved in solid-state NMR experiments of proteins in inhomogeneously disordered oriented lipid bilayers. Using 1H homonuclear decoupling instead of standard 1H heteronuclear decoupling, the 15N line widths may be reduced by up to seven times for such samples. For large oriented membrane proteins, such resolution enhancements may be crucial for assignment and structural interpretation.

Original languageEnglish (US)
Pages (from-to)5028-5029
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number15
DOIs
StatePublished - Apr 16 2008

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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