Resolution and characterization of multiple cytosolic phosphatases capable of hydrolyzing fructose‐1,6‐bisphosphate in spinach and soybean leaves

David M. Pharr, Steven C. Huber

Research output: Contribution to journalArticle

Abstract

The apparent activity of cytoplasmic fructose bisphosphatase (EC 3.1.3.11) in crude extracts of spinach (Spinacia oleracea L.) and soybean (Glycine max [L.] Merr.) leaves was only partially dependent on Mg2+. At least two major non‐chloroplastic fructose bisphosphatases that differed in dependence on Mg2+ were chromatographically resolved from spinach leaves. The Mg2+‐dependent enzyme had an apparent Michaelis constant of 4 μM for fructose‐1,6‐P2, was highly specific, and was strongly inhibited by fructose‐2,6‐P2. Enzyme activity was inhibited by physiological levels of fructose‐6‐P. Both species also contained at least one major enzyme, the activity of which was independent of Mg2+. These enzymes had pH optima near neutrality, Michaelis constants of 25 to 30 μM for fructose‐1,6‐P2, and were inhibited by AMP. Although hexose monophosphates were not metabolized, the enzymes were not specific for fructose‐1,6‐P2: phosphate was released from phosphoenolpyruvate and ribulose‐1, 5‐P2, and with fructose‐1,6‐P2, as substrate, Pi release was about 1.5‐fold greater than fructose‐6‐P production. It is concluded that only the Mg2+‐dependent fructose bisphosphatase, previously characterized, functions in the photosynthetic sucrose formation pathway. Inhibition of the Mg2+‐dependent enzyme by fructose‐6‐P may be involved in regulation of sucrose formation.

Original languageEnglish (US)
Pages (from-to)577-582
Number of pages6
JournalPhysiologia Plantarum
Volume60
Issue number4
DOIs
StatePublished - Apr 1984

Fingerprint

Spinacia oleracea
fructose-bisphosphatase
Soybeans
Phosphoric Monoester Hydrolases
spinach
soybeans
Enzymes
enzymes
Fructose-Bisphosphatase
leaves
sucrose
enzyme activity
Sucrose
hexoses
fructose
Glycine max
Phosphoenolpyruvate
Hexoses
phosphates
Adenosine Monophosphate

Keywords

  • Fruetose bisphosphatase
  • fructose‐2,6‐bisphosphate

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Resolution and characterization of multiple cytosolic phosphatases capable of hydrolyzing fructose‐1,6‐bisphosphate in spinach and soybean leaves. / Pharr, David M.; Huber, Steven C.

In: Physiologia Plantarum, Vol. 60, No. 4, 04.1984, p. 577-582.

Research output: Contribution to journalArticle

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