TY - JOUR
T1 - Resistance to protoporphyrinogen oxidase inhibitors in giant ragweed (Ambrosia trifida)
AU - Faleco, Felipe A.
AU - Machado, Filipi M.
AU - Bobadilla, Lucas K.
AU - Tranel, Patrick J.
AU - Stoltenberg, David
AU - Werle, Rodrigo
N1 - The authors thank the Wisconsin Soybean Marketing Board for funding Felipe Faleco's Graduate Research Assistantship, Bill Verbeten and Jim Stute for collecting and submitting seed samples, and the University of Wisconsin-Madison Cropping Systems Weed Science Program for their technical assistance with glasshouse experiments.
PY - 2024/12
Y1 - 2024/12
N2 - BACKGROUND: Giant ragweed (Ambrosia trifida L.) is one of the most troublesome weed species in corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] cropping systems. Following numerous reports in 2018 of suspected herbicide resistance in several Ambrosia trifida populations from Wisconsin, our objective was to characterize the response of these accessions to acetolactate synthase (ALS), enolpyruvyl shikimate phosphate synthase (EPSPS), and protoporphyrinogen oxidase (PPO) inhibitors applied POST. RESULTS: Four accessions (AT1, AT4, AT6, and AT10) exhibited ≥ 50% plant survival after exposure to the cloransulam 3× rate. Two accessions (AT8 and AT10) and one accession (AT2) exhibited ≥ 50% plant survival after exposure to glyphosate and fomesafen 1× rates, respectively. The AT10 accession exhibited multiple resistance to cloransulam and glyphosate. The AT12 accession was 28.8-fold resistant to fomesafen and 3.7-fold resistant to lactofen. A codon change in PPX2 conferring a R98L substitution was identified as the most likely mechanism conferring PPO-inhibitor resistance. CONCLUSION: To our knowledge, this is the first confirmed case of PPO-inhibitor resistance in Ambrosia trifida globally and we identified the genetic mutation likely conferring resistance. Proactive and diversified integrated weed management strategies are of paramount importance for sustainable long-term Ambrosia trifida management.
AB - BACKGROUND: Giant ragweed (Ambrosia trifida L.) is one of the most troublesome weed species in corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] cropping systems. Following numerous reports in 2018 of suspected herbicide resistance in several Ambrosia trifida populations from Wisconsin, our objective was to characterize the response of these accessions to acetolactate synthase (ALS), enolpyruvyl shikimate phosphate synthase (EPSPS), and protoporphyrinogen oxidase (PPO) inhibitors applied POST. RESULTS: Four accessions (AT1, AT4, AT6, and AT10) exhibited ≥ 50% plant survival after exposure to the cloransulam 3× rate. Two accessions (AT8 and AT10) and one accession (AT2) exhibited ≥ 50% plant survival after exposure to glyphosate and fomesafen 1× rates, respectively. The AT10 accession exhibited multiple resistance to cloransulam and glyphosate. The AT12 accession was 28.8-fold resistant to fomesafen and 3.7-fold resistant to lactofen. A codon change in PPX2 conferring a R98L substitution was identified as the most likely mechanism conferring PPO-inhibitor resistance. CONCLUSION: To our knowledge, this is the first confirmed case of PPO-inhibitor resistance in Ambrosia trifida globally and we identified the genetic mutation likely conferring resistance. Proactive and diversified integrated weed management strategies are of paramount importance for sustainable long-term Ambrosia trifida management.
KW - ALS-inhibitor resistance
KW - PPO-inhibitor resistance
KW - glyphosate resistance
KW - herbicide resistance management
KW - target-site resistance
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U2 - 10.1002/ps.8349
DO - 10.1002/ps.8349
M3 - Article
C2 - 39101354
AN - SCOPUS:85200359571
SN - 1526-498X
VL - 80
SP - 6211
EP - 6221
JO - Pest Management Science
JF - Pest Management Science
IS - 12
ER -