TY - JOUR
T1 - Resistance to multiple temperate and tropical stem and sheath diseases of rice
AU - Rosas, Juan E.
AU - Martínez, Sebastián
AU - Blanco, Pedro
AU - de Vida, Fernando Pérez
AU - Bonnecarrère, Victoria
AU - Mosquera, Gloria
AU - Cruz, Maribel
AU - Garaycochea, Silvia
AU - Monteverde, Eliana
AU - McCouch, Susan
AU - Germán, Silvia
AU - Jannink, Jean Luc
AU - Gutiérrez, Lucía
N1 - Funding Information:
J.E. Rosas, L. Gutiérrez, Dep. of Statistics, College of Agriculture, Univ. de la República, Garzón 780, Montevideo, Uruguay; J.E. Rosas, S. Martínez, P. Blanco, F. Pérez de Vida, National Rice Research Program, National Institute of Agricultural Research (INIA), INIA Treinta y Tres, Villa Sara 33000, Uruguay; V. Bonnecarrère, S. Garaycochea, Biotechnology Unit, INIA, Estación Experimental Wilson Ferreira Aldunate, Rincón del Colorado 90200, Uruguay; G. Mosquera, Rice and Beans Project, CIAT, A.A. 6713, Cali, Colombia; M. Cruz, Latin American Rice Fund, A.A. 6713, Cali, Colombia; E. Monteverde, S. McCouch, Dep. of Plant Breeding and Genetics, Cornell Univ., 240 Emerson Hall, Ithaca, NY 14853; S. Germán, National Research Program on Rainfed Crop Production, INIA, Estación Experimental La Estanzuela, Colonia 70000, Uruguay; J.-L. Jannink, USDA-ARS R.W. Holley Center, Cornell Univ., Ithaca, NY 14853; L. Gutiérrez, Dep. of Agronomy, Univ. of Wisconsin-Madison, 1575 Linden Dr., Madison, WI 53706. Received 29 Mar. 2017. Accepted 19 Sep. 2017. *Corresponding author ([email protected]).
Funding Information:
This study was funded by INIA (Projects AZ-13 and AZ-19). The first author was supported by the Monsanto’s Beachell-Borlaug International Scholarship Program and a fellowship from the Agencia Nacional de Investigación e Innovación (ANII)—Uruguay Grant POS_NAC_2012_1_8627. The authors thank the technical staff of INIA, the Latin American Rice Fund, and CIAT for their valuable support in laboratory, greenhouse, and field work; Stella Avila for pictures of disease symptoms; and two anonymous reviewers for their comments and suggestions that greatly improved the manuscript.
Publisher Copyright:
© Crop Science Society of America.
PY - 2018/3
Y1 - 2018/3
N2 - Stem rot and aggregated sheath spot are the two major stem and sheath diseases affecting rice (Oryza sativa L.) in temperate areas. A third fungal disease, sheath blight, is a major disease in tropical areas. Resistance to these diseases is a key objective in rice breeding programs but phenotyping is challenged by the confounding effects of phenological and morphological traits such as flowering time (FT) and plant height (PH). This study sought to identify quantitative trait loci (QTL) for resistance to these three diseases after removing the confounding effects of FT and PH. Two populations of advanced breeding germplasm, one with 316 tropical japonica and the other with 325 indica genotypes, were evaluated in field and greenhouse trials for resistance to the diseases. Phenotypic means for field and greenhouse disease resistance, adjusted by FT and PH, were analyzed for associations with 29,000 single nucleotide polymorphisms (SNPs) in tropical japonica and 50,000 SNPs in indica. A total of 29 QTL were found for resistance that were not associated with FT or PH. Multilocus models with selected resistance-associated SNPs were fitted for each disease to estimate their effects on the other diseases. A QTL on chromosome 9 accounted for more than 15% of the phenotypic variance for the three diseases. When resistance-associated SNPs at this locus from both the tropical japonica and indica populations were incorporated into the model, resistance was improved for all three diseases with little impact on FT and PH.
AB - Stem rot and aggregated sheath spot are the two major stem and sheath diseases affecting rice (Oryza sativa L.) in temperate areas. A third fungal disease, sheath blight, is a major disease in tropical areas. Resistance to these diseases is a key objective in rice breeding programs but phenotyping is challenged by the confounding effects of phenological and morphological traits such as flowering time (FT) and plant height (PH). This study sought to identify quantitative trait loci (QTL) for resistance to these three diseases after removing the confounding effects of FT and PH. Two populations of advanced breeding germplasm, one with 316 tropical japonica and the other with 325 indica genotypes, were evaluated in field and greenhouse trials for resistance to the diseases. Phenotypic means for field and greenhouse disease resistance, adjusted by FT and PH, were analyzed for associations with 29,000 single nucleotide polymorphisms (SNPs) in tropical japonica and 50,000 SNPs in indica. A total of 29 QTL were found for resistance that were not associated with FT or PH. Multilocus models with selected resistance-associated SNPs were fitted for each disease to estimate their effects on the other diseases. A QTL on chromosome 9 accounted for more than 15% of the phenotypic variance for the three diseases. When resistance-associated SNPs at this locus from both the tropical japonica and indica populations were incorporated into the model, resistance was improved for all three diseases with little impact on FT and PH.
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U2 - 10.3835/plantgenome2017.03.0029
DO - 10.3835/plantgenome2017.03.0029
M3 - Article
C2 - 29505639
AN - SCOPUS:85043773913
SN - 1940-3372
VL - 11
JO - Plant Genome
JF - Plant Genome
IS - 1
M1 - 170029
ER -