Resistance Exercise-induced Regulation of Muscle Protein Synthesis to Intraset Rest

Amadeo F. Salvador, Andrew T. Askow, Colleen F. McKenna, Hsin Yu Fang, Sarah K. Burke, Zhong Li, Alexander V. Ulanov, Scott A. Paluska, Steven J. Petruzzello, Marni D. Boppart, Jonathan M. Oliver, Nicholas A. Burd

Research output: Contribution to journalArticlepeer-review


During a traditional set configuration of resistance exercise (TRD), characterized by a continuous completion of repetitions, a decrease in power output tends to occur throughout a set of repetitions. Inclusion of intraset rest, otherwise known as a cluster set configuration (CLU), counteracts this power decline. However, the effect of a CLU configuration on postexercise myofibrillar protein synthesis rates (MPS) and anabolic signaling has not been investigated. Purpose We aimed to determine if any mechanistic differences exist between TRD and CLU signaling events associated with muscle anabolism. Methods In randomized crossover trials, eight resistance-trained participants (23 ± 1 yr, 81 ± 4.7 kg, body fat: 18% ± 1.9%; 1 repetition maximum [1RM], 150 ± 9.1 kg) performed an acute bout of CLU (4 sets × (2 × 5) repetitions, 30-s intraset rest, 90-s interset rest) and TRD (4 sets × 10 repetitions, 120-s interset rest) barbell back squats at approximately 70% 1RM with total volume load equated during primed continuous l-[ring-13C6]phenylalanine infusions. Blood and muscle biopsy samples were collected at rest and after exercise at 0, 2, and 5 h. Results There was no difference in postexercise MPS between the CLU and TRD condition (P > 0.05) and no changes in phosphorylation of mTORC1 downstream targets (p70S6K and 4EBP1). Total and phosphorylated yes-associated protein on Ser127 transiently increased (P < 0.01) immediately after exercise (t = 0) in CLU (2.1-fold) and TRD condition (2.2-fold). Conclusions Our results show that CLU is a viable anabolic option by preserving power output with similar MPS stimulation when compared with the TRD condition in trained young adults.

Original languageEnglish (US)
Pages (from-to)1022-1030
Number of pages9
JournalMedicine and Science in Sports and Exercise
Issue number5
StatePublished - May 1 2020


  • ERK1/2

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation


Dive into the research topics of 'Resistance Exercise-induced Regulation of Muscle Protein Synthesis to Intraset Rest'. Together they form a unique fingerprint.

Cite this