Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation

Dennis R. Diener, Alice M. Curry, Karl A. Johnson, Benjamin D. Williams, Paul A. Lefebvre, Karen L. Kindle, Joel L. Rosenbaum

Research output: Contribution to journalArticlepeer-review

Abstract

The biflagellate alga Chlamydomonas has been used extensively in the genetic and biochemical analysis of flagellar assembly and motility. We have restored motility to a paralyzed-flagella mutant of Chlamydomonas by transforming with the corresponding wild-type gene. A nitrate reductase-deficient paralyzed-flagella strain, nit1-305 pf-14, carrying mutations in the genes for nitrate reductase and radial spoke protein 3, was transformed with wild-type copies of both genes. Two-thirds of the cells that survived nitrate selection also regained motility, indicating that they had been transformed with both the nitrate reductase and radial spoke protein 3 genes. Transformants typically contained multiple copies of both genes, genetically linked to each other, but not linked to the original mutant loci. Complementation of paralyzed-flagella mutants by transformation is a powerful tool for investigating flagellar assembly and function. (.

Original languageEnglish (US)
Pages (from-to)5739-5743
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number15
DOIs
StatePublished - 1990
Externally publishedYes

Keywords

  • Flagellar genes
  • Motility
  • Radial spokes

ASJC Scopus subject areas

  • General

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