Requirement of Mammalian Timeless for Circadian Rhythmicity

Jessica W. Barnes, Shelley A. Tischkau, Jeffrey A. Barnes, Jennifer W. Mitchell, Penny W. Burgoon, Jason R. Hickok, Martha U. Gillette

Research output: Contribution to journalArticlepeer-review


Despite a central circadian role in Drosophila for the transcriptional regulator Timeless (dTim), the relevance of mammalian Timeless (mTim) remains equivocal. Conditional knockdown of mTim protein expression in the rat suprachiasmatic nucleus (SCN) disrupted SCN neuronal activity rhythms, and altered levels of known core clock elements. Full-length mTim protein (mTim-fl) exhibited a 24-hour oscillation, whereas a truncated isoform (mTIM-s) was constitutivety expressed. mTIM-fl associated with the mammalian clock Period proteins (mPERs) in oscillating SCN cells. These data suggest that mTim is required for rhythmicity and is a functional homolog of dTim on the negative-feedback arm of the mammalian molecular clockwork.

Original languageEnglish (US)
Pages (from-to)439-442
Number of pages4
Issue number5644
StatePublished - Oct 17 2003

ASJC Scopus subject areas

  • General


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