Reproducibility of airway responsiveness in horses using flowmetric plethysmography and histamine bronchoprovocation

R. D. Nolen-Walston, H. Kuehn, R. C. Boston, M. R. Mazan, P. A. Wilkins, S. Bruns, A. M. Hoffman

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Inflammatory airway disease has a high prevalence in horses, but is often a diagnostic challenge. Flowmetric plethysmography and histamine bronchoprovocation (FP/HBP) is a simple and effective tool for diagnosis, but reproducibility of these measurements made over time has not been established. Hypothesis: We hypothesize that the measurement of airway responsiveness in horses using FP/HBP is consistent over both short and long periods of time. Animals: Twenty-nine healthy adult horses from 2 university herds. Methods: In this prospective experimental study, airway responsiveness was determined in each horse at day 0 (baseline [BL]) with FP/ HBP, using PC35 (provocative concentration of histamine needed to increase Dflow by 35%) as a measure of airway responsiveness. Each horse was re-tested 1-4 weeks after BL (short-term [ST]) and again at 3-12 months after BL (longterm [LT]). Results: In the ST period, 23/27 (85%) of the horses had a PC35 that was within 1 doubling concentration of histamine of their BL value, with a mean change of 0.52 doubling concentrations (95% CI 0.26-0.79, range 0-2.06). For the LT data, 19/26 (73%) of horses were within 1 doubling concentration of their BL value, with a mean change of 0.81 doubling concentrations (95% CI 0.45-1.17, range 0.14-3.10). There was no significant difference in reproducibility between the 2 groups of subjects. Conclusions and Clinical Importance: Repeated measurements of airway responsiveness obtained with FP/HBP show acceptable reproducibility over time periods up to a year. However, caution must be used when testing horses when ambient air temperature is low.

Original languageEnglish (US)
Pages (from-to)631-635
Number of pages5
JournalJournal of veterinary internal medicine
Volume23
Issue number3
DOIs
StatePublished - May 2009

Keywords

  • Equine
  • Inflammatory airway disease
  • Lung function test
  • Pulmonary

ASJC Scopus subject areas

  • General Veterinary

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