Replacement of Thymidine by a Modified Base in the Escherichia coli Genome

Angad P. Mehta, Han Li, Sean A. Reed, Lubica Supekova, Tsotne Javahishvili, Peter G. Schultz

Research output: Contribution to journalArticlepeer-review


Prokaryotic and eukaryotic genomic DNA is comprised of the four building blocks A, G, C, and T. We have begun to explore the consequences of replacing a large fraction or all of a nucleoside in genomic DNA with a modified nucleoside. As a first step we have investigated the possibility of replacement of T by 2′-deoxy-5-(hydroxymethyl)uridine (5hmU) in the genomic DNA of Escherichia coli. Metabolic engineering with phage genes followed by random mutagenesis enabled us to achieve approximately 75% replacement of T by 5hmU in the E. coli genome and in plasmids.

Original languageEnglish (US)
Pages (from-to)7272-7275
Number of pages4
JournalJournal of the American Chemical Society
Issue number23
StatePublished - Jun 15 2016
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry


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