Repeated DNA sequences in Triticum (Poaceae): Chromosomal mapping and its bearing on the evolution of B and G genomes

A Lane Rayburn, Bikram S. Gill

Research output: Contribution to journalArticlepeer-review

Abstract

The somatic chromosomes of Triticum turgicum var. durum cv. Langdon and T. dicoccoides (AABB tetraploids), T. timopheevii, and T. araraticum (AAGG tetraploids) were assayed for distribution patterns of a highly repeated 120bp DNA sequence by in situ hybridization. The repeated sequence appears to be an ancient sequence shared with Secale and Aegilops. The distribution patterns of the chromosomes were compared to the patterns of the A and B genome chromosomes of T. aestivum cv. Chinese Spring (AABBDD hexaploid). T. turgidum and T. dicoccoides were observed to have identical in situ hybridization patterns. In both species, nine chromosomes with a total of 21 sites of hybridization were observed. The pattern, with few exceptions, was identical to that of Chinese Spring. T. araraticum and T. timopheevii were observed to have different patterns. In T. araraticum, six chromosomes with 21 total hybridization sites are present while in T. timopheevii nine chromosomes with 19 total sites exist. Major differences in hybridization patterns were observed between the B and G genomes. The divergence of the tetraploid wheats in this study appears to have resulted in changes in location, not in amount, of the ancient repeated sequence.

Original languageEnglish (US)
Pages (from-to)229-235
Number of pages7
JournalPlant Systematics and Evolution
Volume159
Issue number3-4
DOIs
StatePublished - Sep 1988
Externally publishedYes

Keywords

  • Angiosperms Poaceae
  • Evolution
  • G genome chromosomes
  • Triticum
  • biotin labelling
  • in situ hybridization
  • repeated DNA

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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