Release of carboxylating enzymes from maize and sugar cane leaf tissue during progressive grinding

C. Bucke, S. P. Long

Research output: Contribution to journalArticle

Abstract

The release of chlorophyll, chloroplasts, o-diphenols, o-diphenol oxidase activity and carboxylating enzyme activity during the grinding of maize and sugar cane leaf tissue has been correlated with the breakage of different types of cell. Enzymes of the photosynthetic carbon reduction cycle were released in the grinding stage during which the bulk of the mesophyll tissue was disrupted and grana-containing chloroplasts released. Since the largest amount of phenol oxidase activity and of phenols was also released at this stage it is likely that the enzymes were partly inhibited by phenol oxidation products and, therefore, underestimated. PEP carboxylase is released earlier in the grinding process. It is concluded that the photosynthetic carbon reduction cycle enzymes studied are located in mesophyll cell chloroplasts and that the PEP carboxylase resides outside the chloroplasts, either in the cytoplasm of mesophyll cells or in colourless tissue. These results are discussed in relation to current theories regarding the assimilation and shuttling of carbon dioxide in leaves of tropical grasses.

Original languageEnglish (US)
Pages (from-to)199-210
Number of pages12
JournalPlanta
Volume99
Issue number3
DOIs
StatePublished - Sep 1 1971
Externally publishedYes

Fingerprint

Canes
grinding
Chloroplasts
Zea mays
sugarcane
chloroplasts
Mesophyll Cells
mesophyll
Carbon Cycle
corn
Enzymes
enzymes
leaves
Catechol Oxidase
grana
Thylakoids
Monophenol Monooxygenase
carbon
Phenols
monophenol monooxygenase

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

Release of carboxylating enzymes from maize and sugar cane leaf tissue during progressive grinding. / Bucke, C.; Long, S. P.

In: Planta, Vol. 99, No. 3, 01.09.1971, p. 199-210.

Research output: Contribution to journalArticle

@article{0142267a72a94ac1b118763a5d311399,
title = "Release of carboxylating enzymes from maize and sugar cane leaf tissue during progressive grinding",
abstract = "The release of chlorophyll, chloroplasts, o-diphenols, o-diphenol oxidase activity and carboxylating enzyme activity during the grinding of maize and sugar cane leaf tissue has been correlated with the breakage of different types of cell. Enzymes of the photosynthetic carbon reduction cycle were released in the grinding stage during which the bulk of the mesophyll tissue was disrupted and grana-containing chloroplasts released. Since the largest amount of phenol oxidase activity and of phenols was also released at this stage it is likely that the enzymes were partly inhibited by phenol oxidation products and, therefore, underestimated. PEP carboxylase is released earlier in the grinding process. It is concluded that the photosynthetic carbon reduction cycle enzymes studied are located in mesophyll cell chloroplasts and that the PEP carboxylase resides outside the chloroplasts, either in the cytoplasm of mesophyll cells or in colourless tissue. These results are discussed in relation to current theories regarding the assimilation and shuttling of carbon dioxide in leaves of tropical grasses.",
author = "C. Bucke and Long, {S. P.}",
year = "1971",
month = "9",
day = "1",
doi = "10.1007/BF00386838",
language = "English (US)",
volume = "99",
pages = "199--210",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Release of carboxylating enzymes from maize and sugar cane leaf tissue during progressive grinding

AU - Bucke, C.

AU - Long, S. P.

PY - 1971/9/1

Y1 - 1971/9/1

N2 - The release of chlorophyll, chloroplasts, o-diphenols, o-diphenol oxidase activity and carboxylating enzyme activity during the grinding of maize and sugar cane leaf tissue has been correlated with the breakage of different types of cell. Enzymes of the photosynthetic carbon reduction cycle were released in the grinding stage during which the bulk of the mesophyll tissue was disrupted and grana-containing chloroplasts released. Since the largest amount of phenol oxidase activity and of phenols was also released at this stage it is likely that the enzymes were partly inhibited by phenol oxidation products and, therefore, underestimated. PEP carboxylase is released earlier in the grinding process. It is concluded that the photosynthetic carbon reduction cycle enzymes studied are located in mesophyll cell chloroplasts and that the PEP carboxylase resides outside the chloroplasts, either in the cytoplasm of mesophyll cells or in colourless tissue. These results are discussed in relation to current theories regarding the assimilation and shuttling of carbon dioxide in leaves of tropical grasses.

AB - The release of chlorophyll, chloroplasts, o-diphenols, o-diphenol oxidase activity and carboxylating enzyme activity during the grinding of maize and sugar cane leaf tissue has been correlated with the breakage of different types of cell. Enzymes of the photosynthetic carbon reduction cycle were released in the grinding stage during which the bulk of the mesophyll tissue was disrupted and grana-containing chloroplasts released. Since the largest amount of phenol oxidase activity and of phenols was also released at this stage it is likely that the enzymes were partly inhibited by phenol oxidation products and, therefore, underestimated. PEP carboxylase is released earlier in the grinding process. It is concluded that the photosynthetic carbon reduction cycle enzymes studied are located in mesophyll cell chloroplasts and that the PEP carboxylase resides outside the chloroplasts, either in the cytoplasm of mesophyll cells or in colourless tissue. These results are discussed in relation to current theories regarding the assimilation and shuttling of carbon dioxide in leaves of tropical grasses.

UR - http://www.scopus.com/inward/record.url?scp=34250472499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250472499&partnerID=8YFLogxK

U2 - 10.1007/BF00386838

DO - 10.1007/BF00386838

M3 - Article

AN - SCOPUS:34250472499

VL - 99

SP - 199

EP - 210

JO - Planta

JF - Planta

SN - 0032-0935

IS - 3

ER -