Relationships between intracisternal type A and extracellular oncornavirus like particles produced in murine MOPC 460 myeloma cells

D. L. Robertson, P. Yau, D. C. Dobbertin, T. K. Sweeney, S. S. Thach, T. Brendler, R. E. Thach

Research output: Contribution to journalArticlepeer-review

Abstract

Oncornavirus like particles of the ''A'' (both intracisternal and intracytoplasmic) and ''B'' or ''C'' (extracellular) types are produced by murine MOPC 460 myeloma cells. This communication describes a comparative study on intracisternal A and extracellular particles. Both types of particles contain an RNA dependent DNA polymerase activity, traces of 35S and 70S RNA in addition to larger amounts of degraded RNA, and proteins of approximately 76,000 and 45,000 daltons. The 76,000-dalton proteins from intracisternal A and extracellular particles have the same cyanogen bromide peptides. Hybridization kinetic analysis indicates that the RNAs in the two particles are identical or very closely related and share partial homology with Moloney leukemia virus RNA. In contrast, the particles appear to have little or no relationship to murine mammary tumor virus as judged by several different criteria. Electron microscope studies indicate that the extracellular particles arise from the budding of core components through the plasma membrane. These results suggest that the intracisternal A and extracellular oncornavirus like particles produced by MOPC 460 cells are closely related.

Original languageEnglish (US)
Pages (from-to)344-355
Number of pages12
JournalUnknown Journal
Volume18
Issue number1
DOIs
StatePublished - 1976
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Relationships between intracisternal type A and extracellular oncornavirus like particles produced in murine MOPC 460 myeloma cells'. Together they form a unique fingerprint.

Cite this