Relationship of heparin binding with computer-analyzed physical traits of human sperm

D. J. Miller; G. Agnew; W. R. Boone; R. L. Ax; J. M. Vasquez

doi:10.1016/s0015-0282(16)59902-6

Relationship of heparin binding with computer-analyzed physical traits of human sperm

D. J. Miller, G. Agnew, W. R. Boone, R. L. Ax, J. M. Vasquez

Research output: Contribution to journal › Article › peer-review

Abstract

The objective of this study was to determine whether heparin affinity or the concentration of binding sites was related to sperm traits analyzed by a routine computerized semen analysis in human males requesting infertility evaluations. Saturation of heparin binding sites on sperm was achieved in 36 of 50 samples analyzed so that accurate estimates of dissociation constants (Kd) and binding site concentrations could be made. A broad range in Kds (18.2 to 284.5 nM/μg deoxyribonucleic acid [DNA]) and binding site concentrations (2.70 to 44.77 nM/μg DNA) was found. The binding affinity of sperm for heparin was significantly correlated with concentrations of cells in the ejaculate (r = 0.56), concentrations of motile sperm (r = 0.51), percentage of motile sperm (r = 0.33), and total numbers of ejaculated sperm (r = 0.37). The concentration of heparin binding sites also was correlated with concentration of cells in the ejaculate (r = 0.60), concentration of motile sperm (r = 0.50), percentage of motile sperm (r = 0.40), and total number of ejaculated sperm (r = 0.49).

Original language	English (US)
Pages (from-to)	886-892
Number of pages	7
Journal	Fertility and Sterility
Volume	49
Issue number	5
DOIs	https://doi.org/10.1016/s0015-0282(16)59902-6
State	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

Online availability

10.1016/s0015-0282(16)59902-6

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@article{dff2a0a65b3c47658b2f92bcda08672b,

title = "Relationship of heparin binding with computer-analyzed physical traits of human sperm",

abstract = "The objective of this study was to determine whether heparin affinity or the concentration of binding sites was related to sperm traits analyzed by a routine computerized semen analysis in human males requesting infertility evaluations. Saturation of heparin binding sites on sperm was achieved in 36 of 50 samples analyzed so that accurate estimates of dissociation constants (Kd) and binding site concentrations could be made. A broad range in Kds (18.2 to 284.5 nM/μg deoxyribonucleic acid [DNA]) and binding site concentrations (2.70 to 44.77 nM/μg DNA) was found. The binding affinity of sperm for heparin was significantly correlated with concentrations of cells in the ejaculate (r = 0.56), concentrations of motile sperm (r = 0.51), percentage of motile sperm (r = 0.33), and total numbers of ejaculated sperm (r = 0.37). The concentration of heparin binding sites also was correlated with concentration of cells in the ejaculate (r = 0.60), concentration of motile sperm (r = 0.50), percentage of motile sperm (r = 0.40), and total number of ejaculated sperm (r = 0.49).",

author = "Miller, {D. J.} and G. Agnew and Boone, {W. R.} and Ax, {R. L.} and Vasquez, {J. M.}",

note = "Funding Information: Received September 21, 1987; revised and accepted January 18,1988. • Supported by a University of Wisconsin biomedical research support grant (J.M.V.); a University of Wisconsin graduate school grant (J.M.V.); and a consortium grant from Atlantic Breeders Cooperative, NOBA, Sire Power, Inc., and TwentyFirst Century Genetics (R.L.A.). t Department of Dairy Science and Endocrinology-Reproductive-Physiology Program. *D epartment of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility. § Reprint requests: Jaime Vasquez, M.D., Department of Obstetrics and Gynecology, H4/630 CSC, 600 Highland Avenue, Madison, Wisconsin.",

year = "1988",

doi = "10.1016/s0015-0282(16)59902-6",

language = "English (US)",

volume = "49",

pages = "886--892",

journal = "Fertility and Sterility",

issn = "0015-0282",

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T1 - Relationship of heparin binding with computer-analyzed physical traits of human sperm

AU - Miller, D. J.

AU - Agnew, G.

AU - Boone, W. R.

AU - Ax, R. L.

AU - Vasquez, J. M.

N1 - Funding Information: Received September 21, 1987; revised and accepted January 18,1988. • Supported by a University of Wisconsin biomedical research support grant (J.M.V.); a University of Wisconsin graduate school grant (J.M.V.); and a consortium grant from Atlantic Breeders Cooperative, NOBA, Sire Power, Inc., and TwentyFirst Century Genetics (R.L.A.). t Department of Dairy Science and Endocrinology-Reproductive-Physiology Program. *D epartment of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility. § Reprint requests: Jaime Vasquez, M.D., Department of Obstetrics and Gynecology, H4/630 CSC, 600 Highland Avenue, Madison, Wisconsin.

PY - 1988

Y1 - 1988

N2 - The objective of this study was to determine whether heparin affinity or the concentration of binding sites was related to sperm traits analyzed by a routine computerized semen analysis in human males requesting infertility evaluations. Saturation of heparin binding sites on sperm was achieved in 36 of 50 samples analyzed so that accurate estimates of dissociation constants (Kd) and binding site concentrations could be made. A broad range in Kds (18.2 to 284.5 nM/μg deoxyribonucleic acid [DNA]) and binding site concentrations (2.70 to 44.77 nM/μg DNA) was found. The binding affinity of sperm for heparin was significantly correlated with concentrations of cells in the ejaculate (r = 0.56), concentrations of motile sperm (r = 0.51), percentage of motile sperm (r = 0.33), and total numbers of ejaculated sperm (r = 0.37). The concentration of heparin binding sites also was correlated with concentration of cells in the ejaculate (r = 0.60), concentration of motile sperm (r = 0.50), percentage of motile sperm (r = 0.40), and total number of ejaculated sperm (r = 0.49).

AB - The objective of this study was to determine whether heparin affinity or the concentration of binding sites was related to sperm traits analyzed by a routine computerized semen analysis in human males requesting infertility evaluations. Saturation of heparin binding sites on sperm was achieved in 36 of 50 samples analyzed so that accurate estimates of dissociation constants (Kd) and binding site concentrations could be made. A broad range in Kds (18.2 to 284.5 nM/μg deoxyribonucleic acid [DNA]) and binding site concentrations (2.70 to 44.77 nM/μg DNA) was found. The binding affinity of sperm for heparin was significantly correlated with concentrations of cells in the ejaculate (r = 0.56), concentrations of motile sperm (r = 0.51), percentage of motile sperm (r = 0.33), and total numbers of ejaculated sperm (r = 0.37). The concentration of heparin binding sites also was correlated with concentration of cells in the ejaculate (r = 0.60), concentration of motile sperm (r = 0.50), percentage of motile sperm (r = 0.40), and total number of ejaculated sperm (r = 0.49).

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ER -

Relationship of heparin binding with computer-analyzed physical traits of human sperm

Abstract

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