Regulatory genes and environmental regulation of amylovoran biosynthesis in Erwinia amylovora

D. P. Wang, Steven J Clough, Youfu Zhao, G. W. Sundin, S. S. Korban, I. K. Toth

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The requirement of the exopolysaccharide amylovoran for Erwinia amylovora pathogenesis is well documented. However, regulation of amylovoran biosynthesis has not been comprehensively studied. We have previously reported that amylovoran production is strain-dependent in E. amylovora isolates. We have also demonstrated that amylovoran biosynthesis is mainly controlled by the RcsBCD phosphorelay two-component system, which is essential for virulence. In this study, we have systematically characterized a network of regulatory genes as well as various environmental conditions such as pH, temperature, and carbon sources in regulating amylovoran production in vitro. We found that global regulators such as H-NS and accessory proteins of the Rcs system such as RcsF and DjlA negatively regulated amylorovan production in E. amylovora strain Ea1189. In addition, a hypothetical gene (herein named amyR) was also identified that negatively regulated amylovoran production. Our results also indicated that E. amylovora strains produced more amylovoran at high temperature (32°C) or neutral pH (7.0) than at low temperature (22 or °) or acidic pH (5.5). Furthermore, an rcsC mutant strain did not produce higher levels of amylovoran at high temperature than at low temperature, indicating that RcsC may be involved in thermo-regulation of amylovoran production. These results indicate that regulation of amylovoran production is very complicated in E. amylovora and suggest the presence of regulatory networks governing the expression of amylovoran biosynthesis genes.

Original languageEnglish (US)
Title of host publicationXII International Workshop on Fire Blight
PublisherInternational Society for Horticultural Science
Pages195-202
Number of pages8
ISBN (Print)9789066051171
DOIs
StatePublished - May 13 2011

Publication series

NameActa Horticulturae
Volume896
ISSN (Print)0567-7572

Fingerprint

environmental law
Erwinia amylovora
regulator genes
biosynthesis
temperature
exopolysaccharides
thermoregulation
virulence
genes
pathogenesis
mutants
environmental factors
carbon
proteins

Keywords

  • Amylovoran biosynthesis
  • Environmental conditions
  • Gene expression
  • Microarray
  • Regulator
  • Virulence

ASJC Scopus subject areas

  • Horticulture

Cite this

Wang, D. P., Clough, S. J., Zhao, Y., Sundin, G. W., Korban, S. S., & Toth, I. K. (2011). Regulatory genes and environmental regulation of amylovoran biosynthesis in Erwinia amylovora. In XII International Workshop on Fire Blight (pp. 195-202). (Acta Horticulturae; Vol. 896). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2011.896.25

Regulatory genes and environmental regulation of amylovoran biosynthesis in Erwinia amylovora. / Wang, D. P.; Clough, Steven J; Zhao, Youfu; Sundin, G. W.; Korban, S. S.; Toth, I. K.

XII International Workshop on Fire Blight. International Society for Horticultural Science, 2011. p. 195-202 (Acta Horticulturae; Vol. 896).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, DP, Clough, SJ, Zhao, Y, Sundin, GW, Korban, SS & Toth, IK 2011, Regulatory genes and environmental regulation of amylovoran biosynthesis in Erwinia amylovora. in XII International Workshop on Fire Blight. Acta Horticulturae, vol. 896, International Society for Horticultural Science, pp. 195-202. https://doi.org/10.17660/ActaHortic.2011.896.25
Wang DP, Clough SJ, Zhao Y, Sundin GW, Korban SS, Toth IK. Regulatory genes and environmental regulation of amylovoran biosynthesis in Erwinia amylovora. In XII International Workshop on Fire Blight. International Society for Horticultural Science. 2011. p. 195-202. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2011.896.25
Wang, D. P. ; Clough, Steven J ; Zhao, Youfu ; Sundin, G. W. ; Korban, S. S. ; Toth, I. K. / Regulatory genes and environmental regulation of amylovoran biosynthesis in Erwinia amylovora. XII International Workshop on Fire Blight. International Society for Horticultural Science, 2011. pp. 195-202 (Acta Horticulturae).
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abstract = "The requirement of the exopolysaccharide amylovoran for Erwinia amylovora pathogenesis is well documented. However, regulation of amylovoran biosynthesis has not been comprehensively studied. We have previously reported that amylovoran production is strain-dependent in E. amylovora isolates. We have also demonstrated that amylovoran biosynthesis is mainly controlled by the RcsBCD phosphorelay two-component system, which is essential for virulence. In this study, we have systematically characterized a network of regulatory genes as well as various environmental conditions such as pH, temperature, and carbon sources in regulating amylovoran production in vitro. We found that global regulators such as H-NS and accessory proteins of the Rcs system such as RcsF and DjlA negatively regulated amylorovan production in E. amylovora strain Ea1189. In addition, a hypothetical gene (herein named amyR) was also identified that negatively regulated amylovoran production. Our results also indicated that E. amylovora strains produced more amylovoran at high temperature (32°C) or neutral pH (7.0) than at low temperature (22 or °) or acidic pH (5.5). Furthermore, an rcsC mutant strain did not produce higher levels of amylovoran at high temperature than at low temperature, indicating that RcsC may be involved in thermo-regulation of amylovoran production. These results indicate that regulation of amylovoran production is very complicated in E. amylovora and suggest the presence of regulatory networks governing the expression of amylovoran biosynthesis genes.",
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AB - The requirement of the exopolysaccharide amylovoran for Erwinia amylovora pathogenesis is well documented. However, regulation of amylovoran biosynthesis has not been comprehensively studied. We have previously reported that amylovoran production is strain-dependent in E. amylovora isolates. We have also demonstrated that amylovoran biosynthesis is mainly controlled by the RcsBCD phosphorelay two-component system, which is essential for virulence. In this study, we have systematically characterized a network of regulatory genes as well as various environmental conditions such as pH, temperature, and carbon sources in regulating amylovoran production in vitro. We found that global regulators such as H-NS and accessory proteins of the Rcs system such as RcsF and DjlA negatively regulated amylorovan production in E. amylovora strain Ea1189. In addition, a hypothetical gene (herein named amyR) was also identified that negatively regulated amylovoran production. Our results also indicated that E. amylovora strains produced more amylovoran at high temperature (32°C) or neutral pH (7.0) than at low temperature (22 or °) or acidic pH (5.5). Furthermore, an rcsC mutant strain did not produce higher levels of amylovoran at high temperature than at low temperature, indicating that RcsC may be involved in thermo-regulation of amylovoran production. These results indicate that regulation of amylovoran production is very complicated in E. amylovora and suggest the presence of regulatory networks governing the expression of amylovoran biosynthesis genes.

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