Regulation of sucrose-phosphate-synthase activity in spinach leaves by protein level and covalent modification

Joan L. Walker, Steven C. Huber

Research output: Contribution to journalArticle

Abstract

A dot-blot technique was developed using monoclonal antibodies to measure, rapidly and accurately, the amount of sucrose-phosphate synthase (SPS; EC 2.4.1.14) protein present in a crude extract from spinach (Spinacia oleracea L. cv. Dark Green Bloomsdale) leaves; this was compared with SPS activity in this material. During leaf development, increased SPS activity followed closely the increase in enzyme-protein level, indicating denovo synthesis or altered turn-over rates for SPS. In contrast, activation of SPS by illumination of leaves or by mannose treatment of leaf discs in the dark (M. Stitt et al. Planta 174, 217-230) occurred without a significant change in the level of enzyme protein. Since conditions which altered SPS activity did not affect immunoprecipitation or mobility of the 120-kilodalton (kDa) subunit of the enzyme during denaturing gel electrophoresis, some form of protein modification other than proteolysis must be involved. Overall, the results indicate that regulation of SPS activity can involve changes in the level of enzyme protein and-or covalent modification.

Original languageEnglish (US)
Pages (from-to)116-120
Number of pages5
JournalPlanta
Volume177
Issue number1
DOIs
StatePublished - Jan 1 1989

Fingerprint

sucrose-phosphate synthase
Spinacia oleracea
spinach
leaves
Enzymes
Proteins
proteins
enzymes
leaf development
protein subunits
Plantae
mannose
proteolysis
gel electrophoresis
lighting
monoclonal antibodies
Mannose
Lighting
Complex Mixtures
Immunoprecipitation

Keywords

  • Leaf development
  • Light (enzyme activation)
  • Photosynthate partitioning
  • Spinacia (sucrose-phosphate synthase)
  • Sucrose-phosphate synthase

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

Regulation of sucrose-phosphate-synthase activity in spinach leaves by protein level and covalent modification. / Walker, Joan L.; Huber, Steven C.

In: Planta, Vol. 177, No. 1, 01.01.1989, p. 116-120.

Research output: Contribution to journalArticle

Walker, Joan L. ; Huber, Steven C. / Regulation of sucrose-phosphate-synthase activity in spinach leaves by protein level and covalent modification. In: Planta. 1989 ; Vol. 177, No. 1. pp. 116-120.
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