Regulation of sucrose metabolism in higher plants: Localization and regulation of activity of key enzymes

H. Winter, S. C. Huber

Research output: Contribution to journalReview article

Abstract

Sucrose (Suc) plays a central role in plant growth and development. It is a major end product of photosynthesis and functions as a primary transport sugar and in some cases as a direct or indirect regulator of gene expression. Research during the last 2 decades has identified the pathways involved and which enzymes contribute to the control of flux. Availability of metabolites for Suc synthesis and 'demand' for products of sucrose degradation are important factors, but this review specifically focuses on the biosynthetic enzyme sucrose-phosphate synthase (SPS), and the degradative enzymes, sucrose synthase (SuSy), and the invertases. Recent progress has included the cloning of genes encoding these enzymes and the elucidation of posttranslational regulatory mechanisms. Protein phosphorylation is emerging as an important mechanism controlling SPS activity in response to various environmental and endogenous signals. In terms of Suc degradation, invertase-catalyzed hydrolysis generally has been associated with cell expansion, whereas SuSy-catalyzed metabolism has been linked with biosynthetic processes (e.g., cell wall or storage products). Recent results indicate that SuSy may be localized in multiple cellular compartments: (1) as a soluble enzyme in the cytosol (as traditionally assumed); (2) associated with the plasma membrane; and (3) associated with the actin cytoskeleton. Phosphorylation of SuSy has been shown to occur and may be one of the factors controlling localization of the enzyme. The purpose of this review is to summarize some of the recent developments relating to regulation of activity and localization of key enzymes involved in sucrose metabolism in plants.

Original languageEnglish (US)
Pages (from-to)253-289
Number of pages37
JournalCritical Reviews in Biochemistry and Molecular Biology
Volume35
Issue number4
DOIs
StatePublished - Jan 1 2000

Fingerprint

Metabolism
Sucrose
sucrose-phosphate synthase
Enzymes
beta-Fructofuranosidase
Phosphorylation
Degradation
Gene encoding
Plant Development
Photosynthesis
Cloning
Cell membranes
Regulator Genes
Metabolites
Actin Cytoskeleton
Growth and Development
Gene expression
Sugars
Cytosol
Cell Wall

Keywords

  • 14-3-3 proteins
  • Actin cytoskeleton
  • CDPK
  • Invertase
  • Regulatory protein phosphorylation
  • SNF1 protein kinase
  • Sucrose synthase
  • Sucrose-phosphate synthase
  • Sucrose-sensing
  • Translocation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Regulation of sucrose metabolism in higher plants : Localization and regulation of activity of key enzymes. / Winter, H.; Huber, S. C.

In: Critical Reviews in Biochemistry and Molecular Biology, Vol. 35, No. 4, 01.01.2000, p. 253-289.

Research output: Contribution to journalReview article

@article{5ca9844646dd40e4b008733a521fad19,
title = "Regulation of sucrose metabolism in higher plants: Localization and regulation of activity of key enzymes",
abstract = "Sucrose (Suc) plays a central role in plant growth and development. It is a major end product of photosynthesis and functions as a primary transport sugar and in some cases as a direct or indirect regulator of gene expression. Research during the last 2 decades has identified the pathways involved and which enzymes contribute to the control of flux. Availability of metabolites for Suc synthesis and 'demand' for products of sucrose degradation are important factors, but this review specifically focuses on the biosynthetic enzyme sucrose-phosphate synthase (SPS), and the degradative enzymes, sucrose synthase (SuSy), and the invertases. Recent progress has included the cloning of genes encoding these enzymes and the elucidation of posttranslational regulatory mechanisms. Protein phosphorylation is emerging as an important mechanism controlling SPS activity in response to various environmental and endogenous signals. In terms of Suc degradation, invertase-catalyzed hydrolysis generally has been associated with cell expansion, whereas SuSy-catalyzed metabolism has been linked with biosynthetic processes (e.g., cell wall or storage products). Recent results indicate that SuSy may be localized in multiple cellular compartments: (1) as a soluble enzyme in the cytosol (as traditionally assumed); (2) associated with the plasma membrane; and (3) associated with the actin cytoskeleton. Phosphorylation of SuSy has been shown to occur and may be one of the factors controlling localization of the enzyme. The purpose of this review is to summarize some of the recent developments relating to regulation of activity and localization of key enzymes involved in sucrose metabolism in plants.",
keywords = "14-3-3 proteins, Actin cytoskeleton, CDPK, Invertase, Regulatory protein phosphorylation, SNF1 protein kinase, Sucrose synthase, Sucrose-phosphate synthase, Sucrose-sensing, Translocation",
author = "H. Winter and Huber, {S. C.}",
year = "2000",
month = "1",
day = "1",
doi = "10.1080/10409230008984165",
language = "English (US)",
volume = "35",
pages = "253--289",
journal = "Critical Reviews in Biochemistry and Molecular Biology",
issn = "1040-9238",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - Regulation of sucrose metabolism in higher plants

T2 - Localization and regulation of activity of key enzymes

AU - Winter, H.

AU - Huber, S. C.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Sucrose (Suc) plays a central role in plant growth and development. It is a major end product of photosynthesis and functions as a primary transport sugar and in some cases as a direct or indirect regulator of gene expression. Research during the last 2 decades has identified the pathways involved and which enzymes contribute to the control of flux. Availability of metabolites for Suc synthesis and 'demand' for products of sucrose degradation are important factors, but this review specifically focuses on the biosynthetic enzyme sucrose-phosphate synthase (SPS), and the degradative enzymes, sucrose synthase (SuSy), and the invertases. Recent progress has included the cloning of genes encoding these enzymes and the elucidation of posttranslational regulatory mechanisms. Protein phosphorylation is emerging as an important mechanism controlling SPS activity in response to various environmental and endogenous signals. In terms of Suc degradation, invertase-catalyzed hydrolysis generally has been associated with cell expansion, whereas SuSy-catalyzed metabolism has been linked with biosynthetic processes (e.g., cell wall or storage products). Recent results indicate that SuSy may be localized in multiple cellular compartments: (1) as a soluble enzyme in the cytosol (as traditionally assumed); (2) associated with the plasma membrane; and (3) associated with the actin cytoskeleton. Phosphorylation of SuSy has been shown to occur and may be one of the factors controlling localization of the enzyme. The purpose of this review is to summarize some of the recent developments relating to regulation of activity and localization of key enzymes involved in sucrose metabolism in plants.

AB - Sucrose (Suc) plays a central role in plant growth and development. It is a major end product of photosynthesis and functions as a primary transport sugar and in some cases as a direct or indirect regulator of gene expression. Research during the last 2 decades has identified the pathways involved and which enzymes contribute to the control of flux. Availability of metabolites for Suc synthesis and 'demand' for products of sucrose degradation are important factors, but this review specifically focuses on the biosynthetic enzyme sucrose-phosphate synthase (SPS), and the degradative enzymes, sucrose synthase (SuSy), and the invertases. Recent progress has included the cloning of genes encoding these enzymes and the elucidation of posttranslational regulatory mechanisms. Protein phosphorylation is emerging as an important mechanism controlling SPS activity in response to various environmental and endogenous signals. In terms of Suc degradation, invertase-catalyzed hydrolysis generally has been associated with cell expansion, whereas SuSy-catalyzed metabolism has been linked with biosynthetic processes (e.g., cell wall or storage products). Recent results indicate that SuSy may be localized in multiple cellular compartments: (1) as a soluble enzyme in the cytosol (as traditionally assumed); (2) associated with the plasma membrane; and (3) associated with the actin cytoskeleton. Phosphorylation of SuSy has been shown to occur and may be one of the factors controlling localization of the enzyme. The purpose of this review is to summarize some of the recent developments relating to regulation of activity and localization of key enzymes involved in sucrose metabolism in plants.

KW - 14-3-3 proteins

KW - Actin cytoskeleton

KW - CDPK

KW - Invertase

KW - Regulatory protein phosphorylation

KW - SNF1 protein kinase

KW - Sucrose synthase

KW - Sucrose-phosphate synthase

KW - Sucrose-sensing

KW - Translocation

UR - http://www.scopus.com/inward/record.url?scp=0033829914&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033829914&partnerID=8YFLogxK

U2 - 10.1080/10409230008984165

DO - 10.1080/10409230008984165

M3 - Review article

C2 - 11005202

AN - SCOPUS:0033829914

VL - 35

SP - 253

EP - 289

JO - Critical Reviews in Biochemistry and Molecular Biology

JF - Critical Reviews in Biochemistry and Molecular Biology

SN - 1040-9238

IS - 4

ER -