Regulation of insulin-like growth factor-i ribonucleic acid expression, polypeptide secretion, and binding protein activity by growth hormone in porcine preadipocyte cultures

H Rex Gaskins, Jong Won Kim, J. Thomas Wright, Laurie A. Rund, Gary J. Hausman, Gary J. Hausman

Research output: Contribution to journalArticle

Abstract

Insulin-like growth factor-I (IGF-I) is a mito- genic polypeptide postulated to mediate the effect of GH on adipose tissue development. To determine if the effect of GH could be mediated by the local production of IGF-I, we have characterized IGF-I RNA expression, polypeptide secretion, and binding protein activity in primary preadipocyte cultures derived from porcine adipose tissues. GH acutely regulated the abundance of multiple IGF-I RNA transcripts and resulted in a 2fold increase in secreted immunoreactive IGF-I (iIGF-I) poly-peptide in medium conditioned for 48 h by preadipocyte cultures relative to those not receiving GH. Immunocytochemical data indicated that IGF-I is synthesized by presumptive and mature adipocytes. The effect of GH on iIGF-I secretion was observed in cultures derived from both fetal and postnatal animals, while secreted IGF-binding protein activity was increased due to GH only in cultures from fetal animals. The increase in local IGF-I production in response to GH was associated with a decrease in adipocyte development, suggesting that local IGF-I may contribute to suppression of differentiated phenotype.

Original languageEnglish (US)
Pages (from-to)622-630
Number of pages9
JournalEndocrinology
Volume126
Issue number1
DOIs
StatePublished - Jan 1990
Externally publishedYes

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Somatomedins
Insulin-Like Growth Factor I
Swine
RNA
Peptides
Adipocytes
Adipose Tissue
somatotropin-binding protein
Insulin-Like Growth Factor Binding Proteins
Conditioned Culture Medium
Carrier Proteins
Phenotype

ASJC Scopus subject areas

  • Endocrinology

Cite this

Regulation of insulin-like growth factor-i ribonucleic acid expression, polypeptide secretion, and binding protein activity by growth hormone in porcine preadipocyte cultures. / Gaskins, H Rex; Kim, Jong Won; Wright, J. Thomas; Rund, Laurie A.; Hausman, Gary J.; Hausman, Gary J.

In: Endocrinology, Vol. 126, No. 1, 01.1990, p. 622-630.

Research output: Contribution to journalArticle

Gaskins, H Rex ; Kim, Jong Won ; Wright, J. Thomas ; Rund, Laurie A. ; Hausman, Gary J. ; Hausman, Gary J. / Regulation of insulin-like growth factor-i ribonucleic acid expression, polypeptide secretion, and binding protein activity by growth hormone in porcine preadipocyte cultures. In: Endocrinology. 1990 ; Vol. 126, No. 1. pp. 622-630.
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