Regulation of insulin-like growth factor-i ribonucleic acid expression, polypeptide secretion, and binding protein activity by growth hormone in porcine preadipocyte cultures

H. Rex Gaskins, Jong Won Kim, J. Thomas Wright, Laurie A. Rund, Gary J. Hausman, Gary J. Hausman

Research output: Contribution to journalArticlepeer-review

Abstract

Insulin-like growth factor-I (IGF-I) is a mito- genic polypeptide postulated to mediate the effect of GH on adipose tissue development. To determine if the effect of GH could be mediated by the local production of IGF-I, we have characterized IGF-I RNA expression, polypeptide secretion, and binding protein activity in primary preadipocyte cultures derived from porcine adipose tissues. GH acutely regulated the abundance of multiple IGF-I RNA transcripts and resulted in a 2fold increase in secreted immunoreactive IGF-I (iIGF-I) poly-peptide in medium conditioned for 48 h by preadipocyte cultures relative to those not receiving GH. Immunocytochemical data indicated that IGF-I is synthesized by presumptive and mature adipocytes. The effect of GH on iIGF-I secretion was observed in cultures derived from both fetal and postnatal animals, while secreted IGF-binding protein activity was increased due to GH only in cultures from fetal animals. The increase in local IGF-I production in response to GH was associated with a decrease in adipocyte development, suggesting that local IGF-I may contribute to suppression of differentiated phenotype.

Original languageEnglish (US)
Pages (from-to)622-630
Number of pages9
JournalEndocrinology
Volume126
Issue number1
DOIs
StatePublished - Jan 1990
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

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