Abstract

This work describes the use of microfluidic tools to generate covalently immobilized counter gradients of extracellular matrix (ECM) proteins laminin and collagen I. Using these platforms, we demonstrate control of the expression levels of two proteins linked to cell cycle progression by virtue of the spatial location of cells on the gradients, and hence by the local ECM environments in these devices. In contrast to physisorbed gradients, covalently immobilized protein patterns preserved the gradient fidelity, making long term cell studies feasible. This method of precisely controlling local cell environments is simple and broadly portable to other cell types and to other ECM proteins or soluble factors. Our approach promises to enable new investigations in cell biology that will contribute to the establishment of biological design rules for controlling cell growth, differentiation, and function.

Original languageEnglish (US)
Pages (from-to)3061-3068
Number of pages8
JournalLangmuir
Volume21
Issue number7
DOIs
StatePublished - Mar 29 2005

Fingerprint

Extracellular Matrix Proteins
cultured cells
counters
Cytology
Immobilized Proteins
proteins
Proteins
gradients
Cell growth
Laminin
matrices
cells
Microfluidics
Collagen
Cells
collagens
progressions
platforms
cycles

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Regiospecific control of protein expression in cells cultured on two-component counter gradients of extracellular matrix proteins. / Gunawan, Rico C.; Choban, Eric R.; Conour, John E.; Silvestre, Jonathan; Schook, Lawrence B.; Gaskins, H. Rex; Leckband, Deborah E.; Kenis, Paul J.A.

In: Langmuir, Vol. 21, No. 7, 29.03.2005, p. 3061-3068.

Research output: Contribution to journalArticle

@article{6d9afa4c8629466185f1aa9bc66ac594,
title = "Regiospecific control of protein expression in cells cultured on two-component counter gradients of extracellular matrix proteins",
abstract = "This work describes the use of microfluidic tools to generate covalently immobilized counter gradients of extracellular matrix (ECM) proteins laminin and collagen I. Using these platforms, we demonstrate control of the expression levels of two proteins linked to cell cycle progression by virtue of the spatial location of cells on the gradients, and hence by the local ECM environments in these devices. In contrast to physisorbed gradients, covalently immobilized protein patterns preserved the gradient fidelity, making long term cell studies feasible. This method of precisely controlling local cell environments is simple and broadly portable to other cell types and to other ECM proteins or soluble factors. Our approach promises to enable new investigations in cell biology that will contribute to the establishment of biological design rules for controlling cell growth, differentiation, and function.",
author = "Gunawan, {Rico C.} and Choban, {Eric R.} and Conour, {John E.} and Jonathan Silvestre and Schook, {Lawrence B.} and Gaskins, {H. Rex} and Leckband, {Deborah E.} and Kenis, {Paul J.A.}",
year = "2005",
month = "3",
day = "29",
doi = "10.1021/la048303k",
language = "English (US)",
volume = "21",
pages = "3061--3068",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - Regiospecific control of protein expression in cells cultured on two-component counter gradients of extracellular matrix proteins

AU - Gunawan, Rico C.

AU - Choban, Eric R.

AU - Conour, John E.

AU - Silvestre, Jonathan

AU - Schook, Lawrence B.

AU - Gaskins, H. Rex

AU - Leckband, Deborah E.

AU - Kenis, Paul J.A.

PY - 2005/3/29

Y1 - 2005/3/29

N2 - This work describes the use of microfluidic tools to generate covalently immobilized counter gradients of extracellular matrix (ECM) proteins laminin and collagen I. Using these platforms, we demonstrate control of the expression levels of two proteins linked to cell cycle progression by virtue of the spatial location of cells on the gradients, and hence by the local ECM environments in these devices. In contrast to physisorbed gradients, covalently immobilized protein patterns preserved the gradient fidelity, making long term cell studies feasible. This method of precisely controlling local cell environments is simple and broadly portable to other cell types and to other ECM proteins or soluble factors. Our approach promises to enable new investigations in cell biology that will contribute to the establishment of biological design rules for controlling cell growth, differentiation, and function.

AB - This work describes the use of microfluidic tools to generate covalently immobilized counter gradients of extracellular matrix (ECM) proteins laminin and collagen I. Using these platforms, we demonstrate control of the expression levels of two proteins linked to cell cycle progression by virtue of the spatial location of cells on the gradients, and hence by the local ECM environments in these devices. In contrast to physisorbed gradients, covalently immobilized protein patterns preserved the gradient fidelity, making long term cell studies feasible. This method of precisely controlling local cell environments is simple and broadly portable to other cell types and to other ECM proteins or soluble factors. Our approach promises to enable new investigations in cell biology that will contribute to the establishment of biological design rules for controlling cell growth, differentiation, and function.

UR - http://www.scopus.com/inward/record.url?scp=16244366492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16244366492&partnerID=8YFLogxK

U2 - 10.1021/la048303k

DO - 10.1021/la048303k

M3 - Article

C2 - 15779985

AN - SCOPUS:16244366492

VL - 21

SP - 3061

EP - 3068

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 7

ER -