TY - JOUR
T1 - Regenerative growth is constrained by brain tumor to ensure proper patterning in Drosophila
AU - Abidi, Syeda Nayab Fatima
AU - Hsu, Felicity Ting Yu
AU - Smith-Bolton, Rachel K.
N1 - This work was supported by grants R35GM141741 and R01GM107140 from the National Institutes of Health (NIH https://www.nih.gov/) to R.S.-B., and by grant 12-4041 from the Roy J. Carver Charitable Trust (https://www.carvertrust.org/) to R.S.-B. The funders had no role The authors would like to thank Amanda Brock and Sumbul Khan for critical reading of the manuscript and helpful discussions. We also thank Juergen Knoblich, Cheng-Yu Lee, Gary Struhl, Robin Wharton, Nick Sokol, Laura Buttitta, and Cedric Maurange for reagents. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. Transgenic fly stocks were obtained from the Vienna Drosophila Resource Center (VDRC, www.vdrc.at). Antibiodies were obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242.
PY - 2023/12/21
Y1 - 2023/12/21
N2 - Some animals respond to injury by inducing new growth to regenerate the lost structures. This regenerative growth must be carefully controlled and constrained to prevent aberrant growth and to allow correct organization of the regenerating tissue. However, the factors that restrict regenerative growth have not been identified. Using a genetic ablation system in the Drosophila wing imaginal disc, we have identified one mechanism that constrains regenerative growth, impairment of which also leads to erroneous patterning of the final appendage. Regenerating discs with reduced levels of the RNA-regulator Brain tumor (Brat) exhibit enhanced regeneration, but produce adult wings with disrupted margins that are missing extensive tracts of sensory bristles. In these mutants, aberrantly high expression of the pro-growth factor Myc and its downstream targets likely contributes to this loss of cell-fate specification. Thus, Brat constrains the expression of pro-regeneration genes and ensures that the regenerating tissue forms the proper final structure.
AB - Some animals respond to injury by inducing new growth to regenerate the lost structures. This regenerative growth must be carefully controlled and constrained to prevent aberrant growth and to allow correct organization of the regenerating tissue. However, the factors that restrict regenerative growth have not been identified. Using a genetic ablation system in the Drosophila wing imaginal disc, we have identified one mechanism that constrains regenerative growth, impairment of which also leads to erroneous patterning of the final appendage. Regenerating discs with reduced levels of the RNA-regulator Brain tumor (Brat) exhibit enhanced regeneration, but produce adult wings with disrupted margins that are missing extensive tracts of sensory bristles. In these mutants, aberrantly high expression of the pro-growth factor Myc and its downstream targets likely contributes to this loss of cell-fate specification. Thus, Brat constrains the expression of pro-regeneration genes and ensures that the regenerating tissue forms the proper final structure.
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U2 - 10.1371/journal.pgen.1011103
DO - 10.1371/journal.pgen.1011103
M3 - Article
C2 - 38127821
AN - SCOPUS:85180334821
SN - 1553-7390
VL - 19
JO - PLoS genetics
JF - PLoS genetics
IS - 12
M1 - )e1011103
ER -