Reducing the dilution of breath samples for breath hydrogen testing

L. Toresson, J. M. Steiner, D. A. Williams

Research output: Contribution to journalArticlepeer-review


Breath hydrogen testing has a diagnostic potential as a gastrointestinal function test that could be performed in general practice. The purpose of this study was to improve techniques for collection of breath samples and transfer of samples to transport vessels. Breath samples from 10 dogs were collected using both a snug-fitting and a loose-fitting standard anesthetic mask attached to a reservoir bag, and a modified snug-fitting system. CO2 was used as internal standard and mean CO2 concentrations were 1.19 ± 0.76, 2.17 ± 0.66 and 2.68 ± 0.92, respectively. Additional samples were saved in transport tubes for 19 days, during which the hydrogen and carbon dioxide concentrations remained stable. A reliable method for transferring the breath samples from the reservoir bag to vacuum transport tubes was also identified. Our results demonstrate less contamination of breath samples with air than previously reported, and a reproducible method to transfer breath samples to transport vessels.

Original languageEnglish (US)
Pages (from-to)118-122
Number of pages5
JournalResearch in Veterinary Science
Issue number1
StatePublished - Aug 2009
Externally publishedYes


  • Breath hydrogen
  • Gastrointestinal function testing

ASJC Scopus subject areas

  • veterinary(all)


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