Recycling Carbon Dioxide during Xylose Fermentation by Engineered Saccharomyces cerevisiae

Peng Fei Xia, Guo Chang Zhang, Berkley Walker, Seung Oh Seo, Suryang Kwak, Jing Jing Liu, Heejin Kim, Donald R. Ort, Shu Guang Wang, Yong Su Jin

Research output: Contribution to journalArticle

Abstract

Global climate change caused by the emission of anthropogenic greenhouse gases (GHGs) is a grand challenge to humanity. To alleviate the trend, the consumption of fossil fuels needs to be largely reduced and alternative energy technologies capable of controlling GHG emissions are anticipated. In this study, we introduced a synthetic reductive pentose phosphate pathway (rPPP) into a xylose-fermenting Saccharomyces cerevisiae strain SR8 to achieve simultaneous lignocellulosic bioethanol production and carbon dioxide recycling. Specifically, ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum and phosphoribulokinase from Spinacia oleracea were introduced into the SR8 strain. The resulting strain with the synthetic rPPP was able to exhibit a higher yield of ethanol and lower yields of byproducts (xylitol and glycerol) than a control strain. In addition, the reduced release of carbon dioxide by the engineered strain was observed during xylose fermentation, suggesting that the carbon dioxide generated by pyruvate decarboxylase was partially reassimilated through the synthetic rPPP. These results demonstrated that recycling of carbon dioxide from the ethanol fermentation pathway in yeast can be achieved during lignocellulosic bioethanol production through a synthetic carbon conservative metabolic pathway. This strategy has a great potential to alleviate GHG emissions during the production of second-generation ethanol.

Original languageEnglish (US)
Pages (from-to)276-283
Number of pages8
JournalACS synthetic biology
Volume6
Issue number2
DOIs
StatePublished - Feb 17 2017

    Fingerprint

Keywords

  • CO recycling
  • Saccharomyces cerevisiae
  • bioethanol
  • carbon conservation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Xia, P. F., Zhang, G. C., Walker, B., Seo, S. O., Kwak, S., Liu, J. J., Kim, H., Ort, D. R., Wang, S. G., & Jin, Y. S. (2017). Recycling Carbon Dioxide during Xylose Fermentation by Engineered Saccharomyces cerevisiae. ACS synthetic biology, 6(2), 276-283. https://doi.org/10.1021/acssynbio.6b00167