Recombinational repair of DNA damage in Escherichia coli and bacteriophage λ

Research output: Contribution to journalReview articlepeer-review


Although homologous recombination and DNA repair phenomena in bacteria were initially extensively studied without regard to any relationship between the two, it is now appreciated that DNA repair and homologous recombination are related through DNA replication. In Escherichia coli, two-strand DNA damage, generated mostly during replication on a template DNA containing one- strand damage, is repaired by recombination with a homologous intact duplex, usually the sister chromosome. The two major types of two-strand DNA lesions are channeled into two distinct pathways of recombinational repair: daughter- strand gaps are closed by the RecF pathway, while disintegrated replication forks are reestablished by the RecBCD pathway. The phage λ recombination system is simpler in that its major reaction is to link two double-stranded DNA ends by using overlapping homologous sequences. The remarkable progress in understanding the mechanisms of recombinational repair in E. coli over the last decade is due to the in vitro characterization of the activities of individual recombination proteins. Putting our knowledge about recombinational repair in the broader context of DNA replication will guide future experimentation.

Original languageEnglish (US)
Pages (from-to)751-813
Number of pages63
JournalMicrobiology and Molecular Biology Reviews
Issue number4
StatePublished - Dec 1999
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Infectious Diseases


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