Recognition of apoptotic cells by viable cells is specific, ubiquitous, and species independent: Analysis using photonic crystal biosensors

Goutham Pattabiraman, Erich A. Lidstone, Karol Palasiewicz, Brian T. Cunningham, David S. Ucker

Research output: Contribution to journalArticle

Abstract

Apoptotic recognition is innate and linked to a profound immune regulation (innate apoptotic immunity [IAI]) involving anti-inflammatory and immunosuppressive responses. Many of the molecular and mechanistic details of this response remain elusive. Although immune outcomes can be quantified readily, the initial specific recognition events have been difficult to assess. We developed a sensitive, real-time method to detect the recognition of apoptotic cells by viable adherent responder cells, using a photonic crystal biosensor approach. The method relies on characteristic spectral shifts resulting from the specific recognition and dose-dependent interaction of adherent responder cells with nonadherent apoptotic targets. Of note, the biosensor provides a readout of early recognition-specific events in responder cells that occur distal to the biosensor surface. We find that innate apoptotic cell recognition occurs in a strikingly species-independent manner, consistent with our previous work and inferences drawn from indirect assays. Our studies indicate obligate cytoskeletal involvement, although apoptotic cell phagocytosis is not involved. Because it is a direct, objective, and quantitative readout of recognition exclusively, this biosensor approach affords a methodology with which to dissect the early recognition events associated with IAI and immunosuppression.

Original languageEnglish (US)
Pages (from-to)1704-1714
Number of pages11
JournalMolecular biology of the cell
Volume25
Issue number11
DOIs
StatePublished - Jun 1 2014

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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