Recent developments in the application of P450 based biocatalysts

Yifeng Wei; Ee Lui Ang; Huimin Zhao

doi:10.1016/j.cbpa.2017.08.006

Recent developments in the application of P450 based biocatalysts

Yifeng Wei, Ee Lui Ang, Huimin Zhao

Research output: Contribution to journal › Review article › peer-review

Abstract

Cytochrome P450 monooxygenases (P450s) catalyze regioselective and stereoselective oxidative modifications of a wide variety of substrates, and are involved in the biosynthesis of many natural products. Despite the complex requirements of the P450 reaction system and its intransigence to recombinant expression, the promise of harnessing P450 reactivity for the industrial-scale production of specialty chemicals has led to much effort invested in P450 engineering. Here we review recent developments (between 2015 and 2017) in the application of P450s and their engineered variants as biocatalysts. We describe strategies for the reconstitution of P450 activity in heterologous microbial hosts, and the expanding repertoire of non-natural reactions catalyzed by engineered P450s.

Original language	English (US)
Pages (from-to)	1-7
Number of pages	7
Journal	Current Opinion in Chemical Biology
Volume	43
DOIs	https://doi.org/10.1016/j.cbpa.2017.08.006
State	Published - Apr 2018

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

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10.1016/j.cbpa.2017.08.006

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title = "Recent developments in the application of P450 based biocatalysts",

abstract = "Cytochrome P450 monooxygenases (P450s) catalyze regioselective and stereoselective oxidative modifications of a wide variety of substrates, and are involved in the biosynthesis of many natural products. Despite the complex requirements of the P450 reaction system and its intransigence to recombinant expression, the promise of harnessing P450 reactivity for the industrial-scale production of specialty chemicals has led to much effort invested in P450 engineering. Here we review recent developments (between 2015 and 2017) in the application of P450s and their engineered variants as biocatalysts. We describe strategies for the reconstitution of P450 activity in heterologous microbial hosts, and the expanding repertoire of non-natural reactions catalyzed by engineered P450s.",

author = "Yifeng Wei and Ang, {Ee Lui} and Huimin Zhao",

note = "Funding Information: We thank the National Institutes of Health ( GM077596 ) (H.Z.), the GlaxoSmithKline-Singapore Economic Development Board Partnership for Green and Sustainable Manufacturing (E.L.A.), the Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea ( 20153030091450 ) (H.Z.), and the Agency for Science, Research and Technology of Singapore Visiting Investigator Program (H.Z.) for financial support in our development and application of biocatalysis technologies. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2018",

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doi = "10.1016/j.cbpa.2017.08.006",

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AU - Ang, Ee Lui

AU - Zhao, Huimin

N1 - Funding Information: We thank the National Institutes of Health ( GM077596 ) (H.Z.), the GlaxoSmithKline-Singapore Economic Development Board Partnership for Green and Sustainable Manufacturing (E.L.A.), the Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea ( 20153030091450 ) (H.Z.), and the Agency for Science, Research and Technology of Singapore Visiting Investigator Program (H.Z.) for financial support in our development and application of biocatalysis technologies. Publisher Copyright: © 2017 Elsevier Ltd

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N2 - Cytochrome P450 monooxygenases (P450s) catalyze regioselective and stereoselective oxidative modifications of a wide variety of substrates, and are involved in the biosynthesis of many natural products. Despite the complex requirements of the P450 reaction system and its intransigence to recombinant expression, the promise of harnessing P450 reactivity for the industrial-scale production of specialty chemicals has led to much effort invested in P450 engineering. Here we review recent developments (between 2015 and 2017) in the application of P450s and their engineered variants as biocatalysts. We describe strategies for the reconstitution of P450 activity in heterologous microbial hosts, and the expanding repertoire of non-natural reactions catalyzed by engineered P450s.

AB - Cytochrome P450 monooxygenases (P450s) catalyze regioselective and stereoselective oxidative modifications of a wide variety of substrates, and are involved in the biosynthesis of many natural products. Despite the complex requirements of the P450 reaction system and its intransigence to recombinant expression, the promise of harnessing P450 reactivity for the industrial-scale production of specialty chemicals has led to much effort invested in P450 engineering. Here we review recent developments (between 2015 and 2017) in the application of P450s and their engineered variants as biocatalysts. We describe strategies for the reconstitution of P450 activity in heterologous microbial hosts, and the expanding repertoire of non-natural reactions catalyzed by engineered P450s.

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Recent developments in the application of P450 based biocatalysts

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