TY - JOUR
T1 - Recent advances in metabolic engineering of Saccharomyces cerevisiae
T2 - New tools and their applications
AU - Lian, Jiazhang
AU - Mishra, Shekhar
AU - Zhao, Huimin
N1 - Publisher Copyright:
© 2018 International Metabolic Engineering Society
PY - 2018/11
Y1 - 2018/11
N2 - Metabolic engineering aims to develop efficient cell factories by rewiring cellular metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae has been extensively engineered to produce a wide variety of products at high levels from various feedstocks. In this review, we summarize the recent development of metabolic engineering approaches to modulate yeast metabolism with representative examples. Particularly, we highlight new tools for biosynthetic pathway optimization (i.e. combinatorial transcriptional engineering and dynamic metabolic flux control) and genome engineering (i.e. clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) system based genome engineering and RNA interference assisted genome evolution) to advance metabolic engineering in yeast. We also discuss the challenges and perspectives for high throughput metabolic engineering.
AB - Metabolic engineering aims to develop efficient cell factories by rewiring cellular metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae has been extensively engineered to produce a wide variety of products at high levels from various feedstocks. In this review, we summarize the recent development of metabolic engineering approaches to modulate yeast metabolism with representative examples. Particularly, we highlight new tools for biosynthetic pathway optimization (i.e. combinatorial transcriptional engineering and dynamic metabolic flux control) and genome engineering (i.e. clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) system based genome engineering and RNA interference assisted genome evolution) to advance metabolic engineering in yeast. We also discuss the challenges and perspectives for high throughput metabolic engineering.
KW - Genome engineering
KW - Metabolic engineering
KW - Pathway optimization
KW - Saccharomyces cerevisiae
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U2 - 10.1016/j.ymben.2018.04.011
DO - 10.1016/j.ymben.2018.04.011
M3 - Review article
C2 - 29702275
AN - SCOPUS:85046678021
SN - 1096-7176
VL - 50
SP - 85
EP - 108
JO - Metabolic Engineering
JF - Metabolic Engineering
ER -