Recent advances in DNA assembly technologies

Ran Chao, Yongbo Yuan, Huimin Zhao

Research output: Contribution to journalShort surveypeer-review


DNA assembly is one of the most important foundational technologies for synthetic biology and metabolic engineering. Since the development of the restriction digestion and ligation method in the early 1970s, a significant amount of effort has been devoted to developing better DNA assembly methods with higher efficiency, fidelity, and modularity, as well as simpler and faster protocols. This review will not only summarize the key DNA assembly methods and their recent applications, but also highlight the innovations in assembly schemes and the challenges in automating the DNA assembly methods.

Original languageEnglish (US)
Article number12171
JournalFEMS yeast research
Issue number1
StatePublished - 2015


  • Automation
  • Genome synthesis
  • Metabolic engineering
  • Pathway construction
  • Pathway optimization
  • Synthetic biology

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology


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