Reassessment of Dalbulus leafhopper (homoptera: cicadellidae) phylogeny based on mitochondrial DNA sequences

Christopher H Dietrich, S. J. Fitzgerald, J. L. Holmes, W. C. Black IV, L. R. Nault

Research output: Contribution to journalArticle

Abstract

Conflicting hypotheses of phylogenetic relationships among leafhopper species in the genus Dalbulus DeLong have resulted from analyses of morphological and allozyme data. To improve the phylogenetic estimate, nucleotide sequence data from 340 bp of the mitochondrial 12S rDNA gene and 942 bp of the NADH dehydrogenase 1 (ND1) gene were compiled for 10 species of Dalbulus, 1 species of its putative sister group. Baldulus, and 2 outgroups. Intraspecific branch lengths were estimated for Dalbulus gelbus DeLong. Dalbulus maidis (DeLong and Wolcott), and Baldulus tripsaci Kramer and Whitcomb. Sequences were aligned based on secondary structure models of the 12S gene and along codons in the ND1 gene. Phylogenetic analyses of the sequence data using maximum parsimony, genetic distance-neighbor joining and maximum likelihood methods yielded nearly identical results. A test for incongruence indicated that the phylogenetic estimate based on sequence data was not significantly different from that derived based on morphological characters. Allozyme data yielded a topology that was significantly incongruent with both morphological and sequence data. Therefore, the morphological and sequence datasets were combined into a single analysis, which yielded a well-resolved phylogenetic estimate for Dalbulus. The resulting tree supports the hypothesis that the ancestral hosts of Dalbulus were gamagrasses (Tripsacum spp.) and that the acquisition of a preference for maize (Zea mays) arose independently in 2 Dalbulus lineages.

Original languageEnglish (US)
Pages (from-to)590-597
Number of pages8
JournalAnnals of the Entomological Society of America
Volume91
Issue number5
DOIs
StatePublished - Sep 1998

Fingerprint

Dalbulus
Cicadellidae
Homoptera
mitochondrial DNA
nucleotide sequences
phylogeny
NADH dehydrogenase
allozymes
genes
Dalbulus maidis
Tripsacum
codons
topology
genetic distance
Zea mays
corn

Keywords

  • 12S ribosomal DNA
  • Allozymes
  • Dalbulus
  • Morphology
  • NADH dehydrogenase subunit 1
  • mtDNA

ASJC Scopus subject areas

  • Insect Science

Cite this

Reassessment of Dalbulus leafhopper (homoptera : cicadellidae) phylogeny based on mitochondrial DNA sequences. / Dietrich, Christopher H; Fitzgerald, S. J.; Holmes, J. L.; Black IV, W. C.; Nault, L. R.

In: Annals of the Entomological Society of America, Vol. 91, No. 5, 09.1998, p. 590-597.

Research output: Contribution to journalArticle

@article{90f2e52dcce54b33b1e34f0c5f883c72,
title = "Reassessment of Dalbulus leafhopper (homoptera: cicadellidae) phylogeny based on mitochondrial DNA sequences",
abstract = "Conflicting hypotheses of phylogenetic relationships among leafhopper species in the genus Dalbulus DeLong have resulted from analyses of morphological and allozyme data. To improve the phylogenetic estimate, nucleotide sequence data from 340 bp of the mitochondrial 12S rDNA gene and 942 bp of the NADH dehydrogenase 1 (ND1) gene were compiled for 10 species of Dalbulus, 1 species of its putative sister group. Baldulus, and 2 outgroups. Intraspecific branch lengths were estimated for Dalbulus gelbus DeLong. Dalbulus maidis (DeLong and Wolcott), and Baldulus tripsaci Kramer and Whitcomb. Sequences were aligned based on secondary structure models of the 12S gene and along codons in the ND1 gene. Phylogenetic analyses of the sequence data using maximum parsimony, genetic distance-neighbor joining and maximum likelihood methods yielded nearly identical results. A test for incongruence indicated that the phylogenetic estimate based on sequence data was not significantly different from that derived based on morphological characters. Allozyme data yielded a topology that was significantly incongruent with both morphological and sequence data. Therefore, the morphological and sequence datasets were combined into a single analysis, which yielded a well-resolved phylogenetic estimate for Dalbulus. The resulting tree supports the hypothesis that the ancestral hosts of Dalbulus were gamagrasses (Tripsacum spp.) and that the acquisition of a preference for maize (Zea mays) arose independently in 2 Dalbulus lineages.",
keywords = "12S ribosomal DNA, Allozymes, Dalbulus, Morphology, NADH dehydrogenase subunit 1, mtDNA",
author = "Dietrich, {Christopher H} and Fitzgerald, {S. J.} and Holmes, {J. L.} and {Black IV}, {W. C.} and Nault, {L. R.}",
year = "1998",
month = "9",
doi = "10.1093/aesa/91.5.590",
language = "English (US)",
volume = "91",
pages = "590--597",
journal = "Annals of the Entomological Society of America",
issn = "0013-8746",
publisher = "Entomological Society of America",
number = "5",

}

TY - JOUR

T1 - Reassessment of Dalbulus leafhopper (homoptera

T2 - cicadellidae) phylogeny based on mitochondrial DNA sequences

AU - Dietrich, Christopher H

AU - Fitzgerald, S. J.

AU - Holmes, J. L.

AU - Black IV, W. C.

AU - Nault, L. R.

PY - 1998/9

Y1 - 1998/9

N2 - Conflicting hypotheses of phylogenetic relationships among leafhopper species in the genus Dalbulus DeLong have resulted from analyses of morphological and allozyme data. To improve the phylogenetic estimate, nucleotide sequence data from 340 bp of the mitochondrial 12S rDNA gene and 942 bp of the NADH dehydrogenase 1 (ND1) gene were compiled for 10 species of Dalbulus, 1 species of its putative sister group. Baldulus, and 2 outgroups. Intraspecific branch lengths were estimated for Dalbulus gelbus DeLong. Dalbulus maidis (DeLong and Wolcott), and Baldulus tripsaci Kramer and Whitcomb. Sequences were aligned based on secondary structure models of the 12S gene and along codons in the ND1 gene. Phylogenetic analyses of the sequence data using maximum parsimony, genetic distance-neighbor joining and maximum likelihood methods yielded nearly identical results. A test for incongruence indicated that the phylogenetic estimate based on sequence data was not significantly different from that derived based on morphological characters. Allozyme data yielded a topology that was significantly incongruent with both morphological and sequence data. Therefore, the morphological and sequence datasets were combined into a single analysis, which yielded a well-resolved phylogenetic estimate for Dalbulus. The resulting tree supports the hypothesis that the ancestral hosts of Dalbulus were gamagrasses (Tripsacum spp.) and that the acquisition of a preference for maize (Zea mays) arose independently in 2 Dalbulus lineages.

AB - Conflicting hypotheses of phylogenetic relationships among leafhopper species in the genus Dalbulus DeLong have resulted from analyses of morphological and allozyme data. To improve the phylogenetic estimate, nucleotide sequence data from 340 bp of the mitochondrial 12S rDNA gene and 942 bp of the NADH dehydrogenase 1 (ND1) gene were compiled for 10 species of Dalbulus, 1 species of its putative sister group. Baldulus, and 2 outgroups. Intraspecific branch lengths were estimated for Dalbulus gelbus DeLong. Dalbulus maidis (DeLong and Wolcott), and Baldulus tripsaci Kramer and Whitcomb. Sequences were aligned based on secondary structure models of the 12S gene and along codons in the ND1 gene. Phylogenetic analyses of the sequence data using maximum parsimony, genetic distance-neighbor joining and maximum likelihood methods yielded nearly identical results. A test for incongruence indicated that the phylogenetic estimate based on sequence data was not significantly different from that derived based on morphological characters. Allozyme data yielded a topology that was significantly incongruent with both morphological and sequence data. Therefore, the morphological and sequence datasets were combined into a single analysis, which yielded a well-resolved phylogenetic estimate for Dalbulus. The resulting tree supports the hypothesis that the ancestral hosts of Dalbulus were gamagrasses (Tripsacum spp.) and that the acquisition of a preference for maize (Zea mays) arose independently in 2 Dalbulus lineages.

KW - 12S ribosomal DNA

KW - Allozymes

KW - Dalbulus

KW - Morphology

KW - NADH dehydrogenase subunit 1

KW - mtDNA

UR - http://www.scopus.com/inward/record.url?scp=0032427189&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032427189&partnerID=8YFLogxK

U2 - 10.1093/aesa/91.5.590

DO - 10.1093/aesa/91.5.590

M3 - Article

AN - SCOPUS:0032427189

VL - 91

SP - 590

EP - 597

JO - Annals of the Entomological Society of America

JF - Annals of the Entomological Society of America

SN - 0013-8746

IS - 5

ER -