Real-Time Monitoring of Post-Surgical and Post-Traumatic Eye Injuries Using Multilayered Electrical Biosensor Chip

Muhammad S. Khan, Santosh K. Misra, Aaron S. Schwartz-Duval, Enrique Daza, Fatemeh Ostadhossein, Matthew Bowman, Akshay Jain, Gina Taylor, Deana McDonagh, Leanne T. Labriola, Dipanjan Pan

Research output: Contribution to journalArticle

Abstract

Lack of current techniques for the early monitoring of bleb leaks and other post-traumatic or post-surgical ocular injury has posed an unmet clinical need for the development of new techniques. Present evaluation techniques use either subjective or nonquantitative approaches. At present, there are no FDA approved ocular devices that can directly measure ascorbic acid (AA) concentration levels for both tear film (TF) and aqueous humor (AH) at point-of-care (POC) level. Toward this aim, we present a novel POC quantitative assay, called the ocular biosensor device, which can be used to evaluate the integrity of the anterior surface of the eye by measuring the concentration of AA in TF and AH. Herein, we utilize a novel scientific engineering approach for the development of a disposable paper based POC ocular biosensor strip. A grafted poly(styrene)-block-poly(acrylic acid) (PS-b-PAA) and graphene platelet composite with contour based μ-electrodes design (CBμE) exhibit a highly sensitive platform to perform electrochemical immunosensing technique to study clinical samples that have small volumes like tear fluid. Samples used in this study were collected clinically from subjects undergoing therapeutic anterior chamber paracentesis. The proposed biosensor reports the level of AA concentration on an electronic screen, making the results easy to read, efficient, and reliable.

Original languageEnglish (US)
Pages (from-to)8609-8622
Number of pages14
JournalACS Applied Materials and Interfaces
Volume9
Issue number10
DOIs
StatePublished - Mar 15 2017

Keywords

  • aqueous humor
  • ascorbate oxidase
  • ascorbic acid
  • biosensor
  • eye injury
  • glaucoma
  • graphene
  • point-of-care
  • tear film

ASJC Scopus subject areas

  • Materials Science(all)

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    Khan, M. S., Misra, S. K., Schwartz-Duval, A. S., Daza, E., Ostadhossein, F., Bowman, M., Jain, A., Taylor, G., McDonagh, D., Labriola, L. T., & Pan, D. (2017). Real-Time Monitoring of Post-Surgical and Post-Traumatic Eye Injuries Using Multilayered Electrical Biosensor Chip. ACS Applied Materials and Interfaces, 9(10), 8609-8622. https://doi.org/10.1021/acsami.7b01675