Abstract

High-resolution real-time tomography of scattering tissues is important for many areas of medicine and biology. However, the compromise between transverse resolution and depth-of-field, in addition to low sensitivity deep in tissue, continues to impede progress towards cellular-level volumetric tomography. Computed imaging has the potential to solve these long-standing limitations. Interferometric synthetic aperture microscopy is a computed imaging technique enabling high-resolution volumetric tomography with spatially invariant resolution. However, its potential for clinical diagnostics remains largely untapped because full volume reconstructions required lengthy post-processing, and the phase-stability requirements have been difficult to satisfy in vivo. Here, we demonstrate how three-dimensional Fourier-domain resampling, in combination with high-speed optical coherence tomography, can achieve high-resolution in vivo tomography. Enhanced depth sensitivity was achieved over a depth of field extended in real time by more than an order of magnitude. This work lays the foundation for high-speed volumetric cellular-level tomography.

Original languageEnglish (US)
Pages (from-to)444-448
Number of pages5
JournalNature Photonics
Volume7
Issue number6
DOIs
StatePublished - Jun 1 2013

Fingerprint

Optical tomography
Tomography
tomography
high resolution
Tissue
Imaging techniques
Synthetic apertures
high speed
Phase stability
synthetic apertures
Medicine
Microscopic examination
biology
medicine
imaging techniques
Scattering
microscopy
requirements
Processing
sensitivity

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Real-time in vivo computed optical interferometric tomography. / Ahmad, Adeel; Shemonski, Nathan D.; Adie, Steven G.; Kim, Hee Seok; Hwu, Wen Mei W; Carney, P. Scott; Boppart, Stephen A.

In: Nature Photonics, Vol. 7, No. 6, 01.06.2013, p. 444-448.

Research output: Contribution to journalArticle

Ahmad, A, Shemonski, ND, Adie, SG, Kim, HS, Hwu, WMW, Carney, PS & Boppart, SA 2013, 'Real-time in vivo computed optical interferometric tomography', Nature Photonics, vol. 7, no. 6, pp. 444-448. https://doi.org/10.1038/nphoton.2013.71
Ahmad A, Shemonski ND, Adie SG, Kim HS, Hwu WMW, Carney PS et al. Real-time in vivo computed optical interferometric tomography. Nature Photonics. 2013 Jun 1;7(6):444-448. https://doi.org/10.1038/nphoton.2013.71
Ahmad, Adeel ; Shemonski, Nathan D. ; Adie, Steven G. ; Kim, Hee Seok ; Hwu, Wen Mei W ; Carney, P. Scott ; Boppart, Stephen A. / Real-time in vivo computed optical interferometric tomography. In: Nature Photonics. 2013 ; Vol. 7, No. 6. pp. 444-448.
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